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| 流行性乙型脑炎病毒E蛋白的截短表达与间接ELISA诊断方法的建立 | |
| 引用本文： | 祖立闯,王金良,管宇,沈志强,董林,李娇.流行性乙型脑炎病毒E蛋白的截短表达与间接ELISA诊断方法的建立[J].中国预防兽医学报,2010,32(3). |
| 作者姓名： | 祖立闯 王金良 管宇 沈志强 董林 李娇 |
| 作者单位： | 1. 山东绿都生物科技有限公司,山东,滨州256600 2. 山东省滨州畜牧兽医研究院,山东,滨州256600 3. 山东绿都生物科技有限公司,山东,滨州256600;山东省滨州畜牧兽医研究院,山东,滨州256600 |
| 摘 要： | 为建立一种快速检测流行性乙型脑炎病毒抗体的方法,本研究参照已发表的JEV基因组序列,应用RT-PCR扩增了长约1000bp的E基因片段,连接pET30a表达载体中,经诱导后获得了以包涵体形式表达的重组E蛋白。重组蛋白纯化后,经免疫印迹检测证明其具有良好的抗原性和特异性。以该蛋白作为诊断抗原,建立了检测流行性乙型脑炎病毒抗体的E-ELISA诊断方法。该诊断方法具有良好的敏感性、特异性和重复性,为JEV的快速诊断、免疫猪群抗体监测和JEV流行病学调查提供了一种快速、简便的血清学诊断方法。 |
| 关 键 词： | 乙型脑炎病毒 E蛋白 截短表达 间接ELISA |
Development of an indirect ELISA for detecting antibodies to japanese encephalitis virus using recombinant truncated glycoprotein E expressed in E.coli |
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| ZU Li-chuang,WANG Jin-liang,GUAN yu,SHEN Zhi-qiang,DONG Lin,LI Jiao.Development of an indirect ELISA for detecting antibodies to japanese encephalitis virus using recombinant truncated glycoprotein E expressed in E.coli[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(3). | |
| Authors: | ZU Li-chuang WANG Jin-liang GUAN yu SHEN Zhi-qiang DONG Lin LI Jiao |
| Institution: | ZU Li-chuang1,WANG Jin-liang2,GUAN Yu2,SHEN Zhi-qiang1,2,DONG Lin2,LI Jiao1 (1. Sh,ong Lvdu Bio-Sciences , Technology Co.Ltd.,Binzhou 256600,China,2. Sh,ong Binzhou Animal Science , Veterinary Medicine Academy,China) |
| Abstract: | In this study,DNA fragment encoding the Japanese encephalitis virus (JEV) E protein was amplified by RT-PCR,cloned and expressed in E.coli. An indirect ELISA was successfully developed to detect anti-JEV antibody using the purified recombinant E protein as coating antigen. The assay was specific,sensitive and reproducible,which could be used as a rapid serology detection method for monitoring of anti-JEV antibody and epidemiologic survey of JEV. |
| Keywords: | japanese encephalitis virus E protein prokaryotic expression indirect ELISA diagnosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |