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| 恶臭假单胞杆菌转谷氨酰胺酶基因的克隆及原核表达载体的构建 | |
| 引用本文： | 郝学忠,薛飞,朱远茂,马莺,李景鹏.恶臭假单胞杆菌转谷氨酰胺酶基因的克隆及原核表达载体的构建[J].中国预防兽医学报,2005,27(1):21-24. |
| 作者姓名： | 郝学忠 薛飞 朱远茂 马莺 李景鹏 |
| 作者单位： | 1. 东北农业大学,生命科学与生命技术研究中心基因部,黑龙江,哈尔滨,150030 2. 中国农业科学院,哈尔滨兽医研究所,黑龙江,哈尔滨,150001 3. 哈尔滨工业大学,食品学院,黑龙江,哈尔滨,150030 |
| 摘 要： | 通过PCR方法扩增恶臭假单胞杆菌(Pseudomonas putida)H76的转谷氨酰胺酶(transglutaminase,TGase)基因,然后将扩增的约800bp的基因片段克隆到pET-28a( )表达载体上,构建重组TGase的表达载体.酶切鉴定阳性的重组质粒命名为pET-TGase.核苷酸及推导的氨基酸序列分析表明,该基因与恶臭假单胞杆菌KT2440的相应基因有很高的同源性.该载体的构建为进一步研究转谷氨酰胺酶的生物学功能以及应用提供了基础. |
| 关 键 词： | 转谷氨酰胺酶 恶臭假单胞杆菌 基因克隆 表达载体构建 |
| 文章编号： | 1008-0589(2005)06-0021-04 |
| 修稿时间： | 2004年2月19日 |
Cloning and construction of the transglutaminase gene and its prokaryotic expression vector from pseudomonas putida H76 |
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| HAO Xue-zhong,XUE Fei,ZHU Yuan-mao,MA Ying,LI Jing-peng.Cloning and construction of the transglutaminase gene and its prokaryotic expression vector from pseudomonas putida H76[J].Chinese Journal of Preventive Veterinary Medicine,2005,27(1):21-24. | |
| Authors: | HAO Xue-zhong XUE Fei ZHU Yuan-mao MA Ying LI Jing-peng |
| Institution: | HAO Xue-zhong~1,XUE Fei~2,ZHU Yuan-mao~2,MA Ying~3,LI Jing-peng~1 |
| Abstract: | The transglutaminase (TGase) gene of Pseudomonas putida H76 was amplified by polymerase chain reaction(PCR).The amplified fragment that is about 800 bp was cloned into the prokaryotic expression vector pET-28a( ).The recombinant expression vector that contained the target fragment was identified by digestion using restriction endonucleases and named as pET-TGase.The nucletide and deduced amino acid sequences of the cloned transglutaminase gene from Pseudomonas putida H76 were analyzed.The results showed that the gene had a relatively high homology with the corresponding gene from Pseudomonas putida KT2440.The construction of recombinant pET-TGase will provide a basis for the research on biological functions and applications of transglutaminase. |
| Keywords: | transglutaminase pseudomonas putida gene cloning construction of expression vector |
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