| 一株锦鲤疱疹病毒的分离与鉴定 |
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| 引用本文： | 朱霞,李新伟,王好,吕文亮,周井祥.一株锦鲤疱疹病毒的分离与鉴定[J].中国预防兽医学报,2011,33(5). |
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| 作者姓名： | 朱霞 李新伟 王好 吕文亮 周井祥 |
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| 作者单位： | 1. 吉林农业大学动物生产及产品质量安全教育部重点实验室,吉林,长春,130118
2. 吉林农业大学动物科技学院,吉林,长春,130118 |
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| 摘 要： | 本实验对患病鲤鱼进行病毒分离及鉴定,将表现典型锦锂疱疹病毒(KHV)病症状的鲤鱼肾细胞与单层框镜鲤鱼鳍细胞(KFC)共培养,5d～6d后出现典型的KHV细胞病变,即细胞体积增大并出现空泡化;在电镜下观察到典型的KHV病毒粒子;并应用PCR方法扩增获得KHV ORF25基因的849bp基因片段,连接至pMD18-T载体中,经酶切鉴定后序列分析表明,与GenBank登录的3株KHV同源性均为99.9%,命名为KHV-cj.基因进化比较显示,与KHV-J株关系较近.将病毒培养物(10-6.75TCID50)腹腔注射接种实验鱼可使其100%发病,并且症状和病理变化与自然感染KHV的鱼一致,死亡率为75%.表明本研究在国内首次分离到KHV.
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| 关 键 词： | 框镜鲤 锦鲤疱疹病毒 分离鉴定 |
Isolation and identification of Kio herpesvirus |
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| ZHU Xia,LI Xin-wei,WANG Hao,LV Wen-liang,ZHOU Jing-xiang.Isolation and identification of Kio herpesvirus[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(5). |
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| Authors: | ZHU Xia LI Xin-wei WANG Hao LV Wen-liang ZHOU Jing-xiang |
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| Institution: | ZHU Xia1,LI Xin-wei1,WANG Hao1,LV Wen-liang1,ZHOU Jing-xiang2 (1. Key Laboratory of Animal Production,Product Quality and Security,Jilin Agricultural University,Changchun 130118,China,2. College of Animal Science and Technology,China) |
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| Abstract: | A Koi herpesvirus (KHV) was isolated from carp suffering gill necrosis by co-cultivation of the affceted kidney cells with carp fin cells (CFC). Cytopathic effects (CPE) were presented 5 days to 6 days post co-cultivation. The KHV particles were observed under electron microscope by negatively stained the sediment of virus culture with 0.5% phosphotungstate. A 849 bp DNA fragment of ORF25 gene was amplified by PCR and sequencing result indicated that the sequence of nucleotide acid homology was 99.9% with t... |
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| Keywords: | mirror crap Kio herpesvirus isolation and identification |
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