鸡胚中人工感染网状内皮增殖病病毒的检测

为了模仿禽网状内皮增生病毒(REV)垂直感染鸡胚,在1和3日龄的发育SPF鸡胚人工接种不同剂量的REV,并继续孵化至12日龄.在将发育鸡胚制成成纤维细胞单层后,再用抗REV单克隆抗体11B118作间接荧光抗体反应检测REV.结果表明,从所有人工感染REV的鸡胚12日龄制备的成纤维细胞中均能在培养48h检测出REV.该方法可用于检测生产弱毒疫苗的SPF鸡胚及其相应细胞有无REV的污染.

The detection of reticuloendotheliosiv virus (REV)from embryos

To imitate the vertical infectious of chicken embryos with reticuloendotheliosis virus (REV),we inoculated specific-free(SPF)chicken embryo (incubated for 1 and 3 days) with variant doses of REV and then hatch them to day 12.These embryos were used to prepare chicken embryo fibroblasts (CEF)for detection of REV by indirect fluorescence (IFA)with specific monoclonal antibody 11B118.The result showed that REV could be detected 48 hours after culture in all CEF samples prepared from 12-day-old developed embryo inoculated with REV at 1st and 3rd day.This method can be used to detect rev comtaminations in SPF chicken embryos and cef for vaccine production.