| 番鸭细小病毒与鹅细小病毒的PCR鉴别诊断 |
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| 引用本文： | 娄华,杨德威,贺东升. 番鸭细小病毒与鹅细小病毒的PCR鉴别诊断[J]. 中国预防兽医学报, 2000, 22(6): 459-461 |
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| 作者姓名： | 娄华 杨德威 贺东升 |
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| 作者单位： | [1]佛山科技学院动物医学系,南海 [2]华南农业大学动物医学系,广州 |
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| 摘 要： | 通过比较基因ｂａｎｋ中番鸭细小病毒（ＭＤＰＶ）和鹅细小病毒（ＧＰＶ）的全基因序列，针对两种病毒非结构蛋白基因序列的相同区段，分别设计了两对ＰＣＲ引物ＬＨＭＰ／ＬＨＭＰ２和ＬＨＧＰ１／ＬＨＧＰ２，用这两对引物分别对ＭＤＰＶ和ＧＰＶ进行检测，其结果引物ＬＨＭＰ１／ＬＨＭＰ２只特异性地扩增ＭＤＰＶ，而引物ＬＨＧＰ１／ＬＨＧＰ２也只特异性地扩增ＧＰＶ，并且两对引物均扩增出约７２０ｂｐ的长度序列。
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| 关 键 词： | 番鸭 细小病毒 鹅 鉴别诊断 PCR技术 |
| 修稿时间： | 2000-02-14 |
Differential_Diagnosis of Muscovy Duck and Goose Parvoviruses using Polymerase Chain Reaction |
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| Abstract: | By comparing complete nucleotide sequences ofMuscovy duck and Goose parvoviruses registered in the gene bank,two pairs ofprimers(LHMP1/LHGP2和LHGP1/LHGP2)were designed respectively corresponding to the sameregion of non_structural protein gene of the two viruses.MDPV and GPV were detected byusing these two primers.The first primer could only specifically amplify DNA of MDPV whichwere isolated from different areas;and the second primer also could only amplify that ofGPV.However,an 720_bp band all amplified in both cases. |
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