| 抗猪流感病毒HA蛋白单克隆抗体重链基因的克隆及序列分析 |
| |
| 引用本文： | 张新涛,李洪涛,刘明,刘春国,杜金玲.抗猪流感病毒HA蛋白单克隆抗体重链基因的克隆及序列分析[J].中国预防兽医学报,2010,32(2). |
| |
| 作者姓名： | 张新涛 李洪涛 刘明 刘春国 杜金玲 |
| |
| 作者单位： | 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/农业部动物流感参考实验室,黑龙江,哈尔滨,150001 |
| |
| 基金项目： | 国家科技支撑计划项目，黑龙江省自然科学基金 |
| |
| 摘 要： | 为获得抗猪流感病毒HA蛋白单克隆抗体(MAb)重链可变区基因,提取MAb杂交瘤细胞8C4总RNA,通过RT-PCR扩增其重链可变区基因。其DNA序列与GenBank数据库和IMGT/V-QUEST软件比对,序列包含CDR1、CDR2、CDR33个高变区,CDR1含GYTFTSYY 8个氨基酸、CDR2含IYPGDGST 8个氨基酸、CDR3含ARVMIAMDY 9个氨基酸。第22位和96位为骨架区的2个半胱氨酸,形成链内特征性二硫键。该序列符合鼠Ig重链基本框架结构,框架区内无终止密码子,为重排产生的序列。本实验获得的重链序列为研制基因工程抗体奠定了物质基础。
|
| 关 键 词： | 猪流感 重链可变区 序列分析 |
Cloning and sequencing of the variable region fragments encoding the monoclonal antibody against the HA protein of swine influenza virus |
| |
| ZHANG Xin-tao,LI Hong-tao,LIU Ming,LIU Chun-guo,DU Jin-ling.Cloning and sequencing of the variable region fragments encoding the monoclonal antibody against the HA protein of swine influenza virus[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(2). |
| |
| Authors: | ZHANG Xin-tao LI Hong-tao LIU Ming LIU Chun-guo DU Jin-ling |
| |
| Institution: | ZHANG Xin-tao,LI Hong-tao,LIU Ming,LIU Chun-guo,DU Jin-ling (National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Animal Influenza Key Laboratory of Ministry of Agriculture,Harbin 150001,China) |
| |
| Abstract: | The gene encoding heavy chain variable region of a monoclonal antibody (MAb) against the HA protein of swine influenza virus was amplified by RT-PCR from total RNA of the hybridoma cell secreting MAb 8C4. Sequence analysis showed that the VH gene contains 375 nucleotides, encoding 125 amino acids. There are 8 amino acids GYTFTSYY in CDR1, 8 amino acids IYPGDGST in CDR2, and 9 amino acids ARVMIAMDY in CDR3. The 22 and 96 cysteine formed typical disulfide bond in the VH gene. These data provided the foundatio... |
| |
| Keywords: | swine influenza heavy chain variable region sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
