| 广东屠猪肉样品中大肠杆菌耐药性与毒力特征的分析 |
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| 引用本文： | 孙迎,廖晓萍,王秀梅,孙坚,刘宝涛,朱恒乾,张悦,王杨,张美君,刘雅红.广东屠猪肉样品中大肠杆菌耐药性与毒力特征的分析[J].中国预防兽医学报,2011,33(1). |
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| 作者姓名： | 孙迎 廖晓萍 王秀梅 孙坚 刘宝涛 朱恒乾 张悦 王杨 张美君 刘雅红 |
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| 作者单位： | 1. 华南农业大学兽医学院,广东,广州,510642
2. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/细菌病研究室,黑龙江,哈尔滨,150001 |
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| 基金项目： | 国家自然科学基金“广东省政府自然科学基金联合基金重点项目”(U0631006); 广东省自然科学基金创新团队项目(5200638); 教育部创新团队项目(IRT0723) |
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| 摘 要： | 为分析广东地区屠猪肉中大肠杆菌(E.coli)药物敏感菌株的血清型、毒力基因和系统进化背景,本研究从屠猪肉样品中分离出112株E.coli,采用玻片凝集法鉴定血清型,琼脂稀释法测定10种抗菌药的敏感性,PCR方法检测7种毒力相关基因,多重PCR方法进行系统进化背景判定。结果显示,112株E.coli中,定型菌株95株,分别属于15种血清型,其中O65、O131、O8和O158为优势血清型。几乎所有菌株对氟苯尼考、多西环素和四环素高度耐药,而对头孢曲松高度敏感,其中多重耐药菌株多数耐5种以上药物,常见的多重耐药表型是氟苯尼考/氯霉素/多西环素/四环素/氨苄西林。PCR鉴定结果表明,含有毒力基因的菌株中38%至少具有两个毒力基因,其中EAST1+Stx2e和hlyF+Stx2e比较常见。比较常见的毒力基因为Stx2e和EAST1,STb基因仅在一株菌中检测到。多重PCR鉴定结果显示,屠猪肉样品中E.coli主要分布为共生型的A组和B1组。本研究为大肠杆菌病的控制和合理使用抗生素提供实验依据。
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| 关 键 词： | 大肠杆菌 耐药性 血清型 毒力基因 |
Antimicrobial resistance and virulence factors of Escherichia coil isolated from pork in Guangdong provoince |
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| SUN Ying,LIAO Xiao-ping,WANG Xiu-mei,SUN Jian,LIU Bao-tao,ZHU Heng-qian,ZHANG Yue,WANG Yang,ZHANG Mei-jun,LIU Ya-hong.Antimicrobial resistance and virulence factors of Escherichia coil isolated from pork in Guangdong provoince[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(1). |
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| Authors: | SUN Ying LIAO Xiao-ping WANG Xiu-mei SUN Jian LIU Bao-tao ZHU Heng-qian ZHANG Yue WANG Yang ZHANG Mei-jun LIU Ya-hong |
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| Institution: | SUN Ying1,LIAO Xiao-ping1,WANG Xiu-mei2,SUN Jian1,LIU Bao-tao1,ZHU Heng-qian1,ZHANG Yue1,WANG Yang1,ZHANG Mei-jun1,LIU Ya-hong1*(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China,2.Division of Bacterial Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Resaerch Institute,Chinese Academy of Agricultural Science,Harbin 150001,China) |
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| Abstract: | This study investigated the prevalence of antimicrobial resistance serotypes,virulence genes and phylogenetic background of 112 Escherichia coil isolates from pork in Guangdong area.The O serotypes were identified by slide agglutination tests,the resistance phenotypes were tested by agar dilution.All isolates were screened for virulence genes by PCR,and the phylogenetic background determinated by multiplex PCR.Fifteen O serotypes were identified from 95 E.coli isolates,of which O65,O131,O8 and O158 were dom... |
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| Keywords: | Escherichia coil resistance serotypes virulence genes |
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