| 表达鸡传染性支气管炎病毒S1基因重组腺病毒的构建 |
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| 引用本文： | 耿合员,孙元,韩宗玺,邵昱昊,仇华吉,孔宪刚,刘胜旺.表达鸡传染性支气管炎病毒S1基因重组腺病毒的构建[J].中国预防兽医学报,2011,33(3). |
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| 作者姓名： | 耿合员 孙元 韩宗玺 邵昱昊 仇华吉 孔宪刚 刘胜旺 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨,150001 |
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| 基金项目： | 国家十一五科技支撑计划重大资助项目(2006BAD06A03); 公益性行业(农业)科研专项经费项目(200803020) |
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| 摘 要： | 为构建表达鸡传染性支气管炎病毒(IBV)主要免疫原S1蛋白的重组腺病毒,本研究以复制缺陷型人5型腺病毒为载体,以IBVCK/CH/LHLJ/04V株S1基因为外源插入靶基因,通过细菌内同源重组法构建了一株稳定表达IBVS1蛋白(90ku)的重组腺病毒,命名为rAdV-S1。通过PCR鉴定、间接免疫荧光及western blot检测证实S1蛋白在重组腺病毒中获得表达。对构建的重组腺病毒和亲本腺病毒的生长动力学分析表明,该重组病毒的毒价为108.25TCID50/mL,并且两者在生长动力学方面无显著差异。本研究为动物试验和该重组腺病毒免疫特性的研究奠定基础。
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| 关 键 词： | 禽传染性支气管炎病毒 S1基因 重组腺病毒 |
Construction of recombinant adenovirus expressing S1 subunit of spike protein of infectious bronchitis virus |
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| GENG He-yuan,SUN Yuan,HAN Zong-xi,SHAO Yu-hao,QIU Hua-ji,KONG Xian-gang,LIU Sheng-wang.Construction of recombinant adenovirus expressing S1 subunit of spike protein of infectious bronchitis virus[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(3). |
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| Authors: | GENG He-yuan SUN Yuan HAN Zong-xi SHAO Yu-hao QIU Hua-ji KONG Xian-gang LIU Sheng-wang |
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| Institution: | GENG He-yuan,SUN Yuan,HAN Zong-xi,SHAO Yu-hao,QIU Hua-ji,KONG Xian-gang,LIU Sheng-wang*(Division of Avian Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | The S1 subunit of spike protein(90 ku) of infectious bronchitis virus(IBV) is the main immunodominant protein which can induce virus neutralization and haemagglutination-inhibition antibodies.In this study,a recombinant replication-defective human 5 adenovirus(rAdV-S1) that expresses S1 gene from IBV strain CK/CH/LHLJ/04V was constructed with viral titer of 108.25 TCID50/mL in HEK293.Western blot and indirect immunofluorescence assay indicated that the S1 gene was expressed in HEK293 cells infected with rAd... |
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| Keywords: | infectious bronchitis virus S1 gene recombinant adenovirus |
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