检测猪链球菌２型的溶菌酶释放蛋白（ＭＲＰ）的ＰＣＲ方法的建立

根据猪链球菌２型的mrp基因自设计和合成了一对可扩增长度为８８５bp目的片段的引物，成功地建立了检测溶菌酶释放蛋白（ＭＰＲ）的ＰＣＲ方法，用ＸbaⅠ内切酶进行酶切，获得了与预期一致的５７8bp和３０７bp２的２个片段，并进行了ＰＣＲ的特异性试验和敏感性试验。对马链球菌兽疫亚种、猪丹毒杆菌、猪肺疫巴氏杆菌和猪肺炎支原体的ＰＣＲ检测结果均呈阴性；检测的敏感度可达１００个细菌。另外，对９株从病猪体内分离的猪链球菌２型菌株进行检测了８个呈阳性；对１５份正常屠宰猪扁桃体分离物的检测结果是１份为阳性。结果表明此法特异性敏感性均很高，可作为ＭＲＰ快速诊和流行病学调查的手段。

Detection of MRP of Streptococcus Suis Type 2 by PCR

A PCR assay for the rapid and sensitive detection of MRP of Streptococcus suis type 2 was developed.The PCR primers based on the MRP gene of S.suis type 2 could extend a 885bp PCR product.With Xba I digestion,the PCR product could produce a 578bp and a 307bp DNA fragment as expected.Using the same pairs no PCR product was detected from S.equisimilis,E.rhusiopathiae,P.pneumotropica, and M.hyopneumoniae.The PCR could detect 100 bacteria.Of 9 strains of S. suis type 2 isolated from diseased pigs,8 were positive.Of 15 porcine tonsillar specimens,1 was positive.The results demonstrated that the PCR is a highly specific and sensitive diagnostic tool for the detection of MRP from S.suis type 2 and can be used for fast diagnosis and epidemic survey.