| 一株鸡源H6N1亚型禽流感病毒全基因的分子特征 |
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| 引用本文： | 刘丽玲,李雁冰,施建忠,田国彬,张翼,曾显营,刘娣,陈化兰.一株鸡源H6N1亚型禽流感病毒全基因的分子特征[J].中国预防兽医学报,2010,32(3). |
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| 作者姓名： | 刘丽玲 李雁冰 施建忠 田国彬 张翼 曾显营 刘娣 陈化兰 |
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| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室/兽医生物技术国家重点实验室黑龙江省农业科学院,黑龙江,哈尔滨150086
2. 中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室/兽医生物技术国家重点实验室 |
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| 基金项目： | 农业部专项.动物流感防治与监测 |
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| 摘 要： | 2008年国家禽流感参考实验室在我国禽流感流行病学调查期间分离到1株鸡源H6N1亚型禽流感病毒(AIV)A/Chicken/ZheJiang/80/2008(H6N1)(简称为CK/ZJ/80/08),为了弄清该病毒的分子特征,我们对其8个基因片段分别进行扩增和序列测定,对每个基因进行BLAST分析,找出同源性最高的毒株。利用DNAStar中的Megalign功能进行进化分析。结果表明CK/ZJ/80/08的HA裂解位点附近的氨基酸序列为QIETR↓GLF,推测可能为一株低致病力AIV。其HA基因与日本北海道的A/duck/Hokkaido/228/2003(H6N8)和黑龙江的A/mallard/Heilongjiang/131/2006(H6N2)以及香港早期分离株A/chicken/HongKong/17/77(H6N1)等处于同一分支;NA基因在颈部没有缺失,与A/duck/Tsukuba/718/2005(H1N1)、A/goose/Guangdong/1/96(H5N1)等处于同一分支;M基因与A/duck/Hokkaido/W90/2007(H10N7)高度同源(同源性为99%);NS基因与A/duck/Denmark/65047/04(H5N2)和A/goose/Guangdong/1/96(H5N1)处于同一分支。NP、PA、PB1、PB2分别与贵州和江西分离的H5N2亚型AIV的相应基因关系密切,同源性分别为98%、97%、97%、97%。由此推测CK/ZJ/80/08可能是由H6N2、H1N1、H10N7、H5N2等多个亚型病毒重组而成。
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| 关 键 词： | 禽流感病毒 H6N1亚型 全基因 分子特征 |
Molecular characterizations of a isolated from chicken H6N1 subtype avian influenza virus |
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| LIU Li-ling,LI Yan-bing,SHI Jian-zhong,TIAN Guo-bin,ZHANG Yi,ZENG Xian-ying,LIU Di,CHEN Hua-lan.Molecular characterizations of a isolated from chicken H6N1 subtype avian influenza virus[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(3). |
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| Authors: | LIU Li-ling LI Yan-bing SHI Jian-zhong TIAN Guo-bin ZHANG Yi ZENG Xian-ying LIU Di CHEN Hua-lan |
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| Institution: | LIU Li-ling1,2,LI Yan-bing1,SHI Jian-zhong1,TIAN Guo-bin1,ZHANG Yi1,ZENG Xian-ying1,LIU Di2,CHEN Hua-lan1 (1. Animal Influenza Laboratory of the Ministry of Agriculture , State Key Labotory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China,2. Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China) |
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| Abstract: | The H6N1 subtype of avian influenza virus(AIV) CK/ZJ/80/08 (H6N1) was isolated from a chicken during an epidemiological survillence in 2008.In this study,the full genome of AIV CK/ZJ/80/08 was sequenced and analyzed.The results showed that the CK/ZJ/80/08 strain had a HA cleavage site of QIETg ↓ GLF,which is a characteristic structure of low pathogenesis AIV.The HA gene sequence was closely related to A/duck/Hokkaido/228/2003(H6N8),A/mallard/Heilongjiang/131/2006 (H6N2) and A/chicken/HongKong/17/77 (H6N1).The NA genes of CK/ZJ/80/08,A/duck/Tsukuba/718/2005 (HINl) and A/Goose/Guangdong/1/96 (H5N1) shared the same phylogenetic lineage.The M gene had the highest homology with that of A/duck/Hokkaido/W90/2007 (H10N7),and NS genes of CK/ZJ/80/08,A/duck/Denmark/65047/04 (H5N2) and A/Goose/Guangdong/1/96 (H5N1) also shared same lineage.The NP,PA,PBI and PB2 of CK/ZJ/80/08 was closely related to that of H5N2 subtype AIV isolated from Jiangxi province.These data showed that CK/ZJ/80/08 was possibly a reassortant virus derived from H6N2,H1N1,H1ON7 and H5N2 subtypes AIV. |
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| Keywords: | Avian Influenza virus H6N1 subtype molecular characterization |
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