| 牛海绵状脑病几种免疫学检测方法的比较 |
| |
| 引用本文： | 王志亮 邹艳丽 R.Meyer 谢仲伦 管恩平 宋翠平 徐天刚 郑东霞 杜文金 滕翔雁 肖肖 王树双 A.Zurbringgen,M.Vandevelde.牛海绵状脑病几种免疫学检测方法的比较[J].中国动物检疫,2001,18(5):24-27. |
| |
| 作者姓名： | 王志亮 邹艳丽 R.Meyer 谢仲伦 管恩平 宋翠平 徐天刚 郑东霞 杜文金 滕翔雁 肖肖 王树双 A.Zurbringgen M.Vandevelde |
| |
| 作者单位： | 农业部动物检疫所青岛 266032 |
| |
| 摘 要： | 本文对免疫组化、免疫转印（Western-blotting)和酶联免疫吸附试验（ELISA）3种牛海绵状脑病（RSE）免疫学检测方法进行了综合比较。通过比较发现免疫组化及免疫转印法与标准品真实情况的符合率均为100%，ELISA的符合率稍差。免疫组化法操作简单、结果容易判断、价格低廉，是较适合我国使用的检测方法，用该方法对我国部分样品进行检测，结果均为阴性。
|
| 关 键 词： | 牛海绵状脑病 免疫组织化学 免疫转印 酶联免疫吸附试验 朊病毒 |
| 文章编号： | 1005-994X(2001)05-0024-04 |
Hemagglutination and Hemagglutination Inhibition Test of Equine Viral Arteritis |
| |
| Wang Zhiliang,Zou Yanli,R.Meyer,Xie Zhonglun,Guan Enping,Song Cuiping,Xu Tiangang,Zheng Dongxi,Du Wejin,Teng Xiangyan,Xiao Xiao,Wang Shushuang,A.Zurbriggen,M.Vandevelde.Hemagglutination and Hemagglutination Inhibition Test of Equine Viral Arteritis[J].China Journal Of Animal Quarantine,2001,18(5):24-27. |
| |
| Authors: | Wang Zhiliang Zou Yanli RMeyer Xie Zhonglun Guan Enping Song Cuiping Xu Tiangang Zheng Dongxi Du Wejin Teng Xiangyan Xiao Xiao Wang Shushuang AZurbriggen MVandevelde |
| |
| Abstract: | Three immuno -assays: immunohisto - chemistry, Western-blotting and enzyme - liked absorbent assay (ELISA) were compared for the detection of bovine sponiform encephalopathy (BSE) .It was found that the coinci- deuce of immunohistochemistry and western blotting with the true positive negative cases is 100%, and the co- incidence of ELISA is somewhat lower.The immunohisto - chemistry assay is simple in operation, and easy in judge- ment with low cost,and is well suit to China's condition for detection of the disease.52 samples were collected and detected by using the method call with negative results. |
| |
| Keywords: | Bovine sponiform encephalopathy immunohistochemistry assay Western blotting ELISA Prion |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国动物检疫》浏览原始摘要信息 |
| 点击此处可从《中国动物检疫》下载免费的PDF全文 |
