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| 结核分枝杆菌疫源追踪的基因检测分析 | |
| 引用本文： | 万学勤,曾方银,唐冬生,李万可兰,蒋泓,翁闪凡,黄永芬.结核分枝杆菌疫源追踪的基因检测分析[J].中国动物检疫,2010,27(1):43-44. |
| 作者姓名： | 万学勤 曾方银 唐冬生 李万可兰 蒋泓 翁闪凡 黄永芬 |
| 作者单位： | 1. 佛山科学技术学院医学院检药系,广东佛山,528000 2. 南方医科大学南方医院检验科 3. 东北大学中荷生物医学信息与工程学院 4. 华南农业大学动物遗传育种与繁殖 |
| 基金项目： | 佛山市科技局医学攻关项目 |
| 摘 要： | 目的探讨牛源性人结核疫源追踪的基因分析方法。方法采用复方PCR结核杆菌快速鉴定技术,针对特征性分枝杆菌标志基因(MTP40基因、α抗原基因和IS6110),对结核病人和患牛的标本,进行检测。结果患者标本结核分枝杆菌阳性;患牛标本牛分枝杆菌和结核分枝杆菌分别阳性。结论结核病奶牛不仅是人类牛型结核的可能来源,也可能成为人类人型结核的潜在来源。 |
| 关 键 词： | 核病 传染源 PCR 牛乳 |
Genetic testing and analysis for Mycobacterium tuberculosis foci tracking |
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| WAN Xueqin,ZENG Fangyin,TANG Dongsheng,LI Wankelan,JIANG Hong,WENG Shanfan,HUANG Yongfen.Genetic testing and analysis for Mycobacterium tuberculosis foci tracking[J].China Journal Of Animal Quarantine,2010,27(1):43-44. | |
| Authors: | WAN Xueqin ZENG Fangyin TANG Dongsheng LI Wankelan JIANG Hong WENG Shanfan HUANG Yongfen |
| Institution: | 1.Medical College of Guangdong Foshan University, Foshan 528000, China;2.Nanfang Hospital, Nanfang Medical University;3.Sino-Dutch Biomedical and Information Engineering School Of Northeastern University;4.South China Agricultral University) |
| Abstract: | Objective To investigate the use of genetic analysis to study the infection source of tuberculosis. Method Compound PCR for the rapid identification of Mycobacterium characteristic marker genes(MTP40 gene, α antigen gene and IS6110), was used to test specimens from human and bovine TB cases. Results The specimens from human TB cases were positive for Mycobacterium tuberculosis, and the specimens from bovine TB cases were positive for Mycobacterium bovis and Mycobacterium tuberculosis respectively. Conclusion Dairy cattle TB cases are not only the possible source of human infection with Mycobacterium bovis, but also the possible source of human infection with Mycobacterium tuberculosis. |
| Keywords: | PCR |
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