| 聚丙烯酰胺凝胶电泳法鉴定禽呼肠孤病毒 |
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| 引用本文： | 单松华,刘学忠.聚丙烯酰胺凝胶电泳法鉴定禽呼肠孤病毒[J].中国动物检疫,1993(2):13-14. |
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| 作者姓名： | 单松华 刘学忠 |
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| 作者单位： | 上海动植物检疫局 200032
(单松华)，上海动植物检疫局 200032(刘学忠) |
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| 摘 要： | 从感染禽呼肠孤病毒的细胞中,以SDS和酚提取病毒核酸,通过聚丙烯酰胺凝胶电泳(PAGE)法将分节段的核酸分开,经银染色显示,呈3-3-1-3分布,此法可用于禽呼肠孤病毒的快速检测、鉴定。同样方法未能使传染性法氏囊病毒核酸显示。
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| 关 键 词： | 禽呼肠孤病毒 传染性法氏囊病毒 核酸提取 聚丙烯酰胺凝胶电泳 |
Polyacrylamide Gel Electrophoresis for the Detection of Avian Reovirus |
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| Shan Song-hua and Liu Xue-zhong.Polyacrylamide Gel Electrophoresis for the Detection of Avian Reovirus[J].China Journal Of Animal Quarantine,1993(2):13-14. |
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| Authors: | Shan Song-hua and Liu Xue-zhong |
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| Institution: | Shanghai Animal and Plant Quarantine Bureau 200032 |
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| Abstract: | The viral nucleic acid was extracted with SDS and phenol from cells infected with avian reovirus and segmented by polyacrylamide gel electrophoresis. The segments showed 3-3-1-3 pattern distriution after silver-staining. The method could be used in rapid detection and identification of avian reovirus. The same precedure failed to demonstrate the nucleic acid of infectious bursal disease virus. |
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| Keywords: | PAGE Avian reovirus |
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