| 非洲猪瘟病毒实时荧光定量PCR检测方法的建立 |
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| 引用本文： | 黄萍,肖家勇,欧阳振宇,陈盼,白雪,朱中武,孟芳,吴愈柏,唐连飞.非洲猪瘟病毒实时荧光定量PCR检测方法的建立[J].中国动物检疫,2010,27(11):28-30. |
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| 作者姓名： | 黄萍 肖家勇 欧阳振宇 陈盼 白雪 朱中武 孟芳 吴愈柏 唐连飞 |
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| 作者单位： | 湖南出入境检验检疫局检验检疫技术中心;湖南出入境检验检疫局动物检疫处 |
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| 摘 要： | 根据GenBank公布的非洲猪瘟病毒(ASFV)P72基因序列,设计了一对特异性引物和探针,使用含有选定检测序列的重组质粒标准品绘制标准曲线,建立了非洲猪瘟病毒实时荧光定量PCR检测方法。结果表明:该方法的敏感性可达100个拷贝值,具有良好的敏感性和重复性。
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| 关 键 词： | 非洲猪瘟病毒 P72基因 实时荧光定量PCR 质粒 |
Development of a real-time fluorescent quantitative PCR method for rapid detection of African swine fever virus |
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| Huang Ping,Xiao Jiayong,OuYang Zhenyu,Chen Pan,Bai Xue,Zhu Zhongwu,Meng Fang,Wu Yubai,Tang Lianfei.Development of a real-time fluorescent quantitative PCR method for rapid detection of African swine fever virus[J].China Journal Of Animal Quarantine,2010,27(11):28-30. |
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| Authors: | Huang Ping Xiao Jiayong OuYang Zhenyu Chen Pan Bai Xue Zhu Zhongwu Meng Fang Wu Yubai Tang Lianfei |
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| Institution: | 1(1.Inspection and Quarantine Technology Center,Hunan Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China,Changsha,410004,China,2.Department of Animal Quarantine,Hunan Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China,Changsha,410004,China) |
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| Abstract: | A pair of specific primers and probe were designed according to the published sequence of P72 gene of African swine fever virus(ASFV)in GenBank and a series of dilutions of recombinant plasmids were prepared and used to generate standard curves,to establish a real-time fluorescent quantitative PCR method for detection of ASFV.The results showed that the PCR method was capable to detect 100 copies of African swine fever virus.The method was very sensitive and repeatable. |
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| Keywords: | African swine fever virus P72 gene real-time fluorescence quantitative PCR plasmids |
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