| 3A蛋白间接ELISA试验检测猪口蹄疫感染抗体的研究 |
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| 引用本文： | 龚振华,蒋正军,王若聪,胡永浩,刘俊辉,陈书琨,董自珍,祁会彩,管远红,王玉东,郑增忍.3A蛋白间接ELISA试验检测猪口蹄疫感染抗体的研究[J].中国动物检疫,2005,22(11):20-22. |
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| 作者姓名： | 龚振华 蒋正军 王若聪 胡永浩 刘俊辉 陈书琨 董自珍 祁会彩 管远红 王玉东 郑增忍 |
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| 作者单位： | [1]农业部动物检疫所,青岛266032 [2]甘肃农业大学,青岛266032 [3]深圳质量监督检验检疫局,青岛266032 [4]天津质量监督检验检疫局,青岛266032 |
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| 摘 要： | 用表达的口蹄疫3A蛋白作为包被抗原，建立了间接ELISA试验，对口蹄疫病毒感染血清、免疫血清和其它非口蹄疫病毒血清进行了检测研究。结果表明，3A蛋白间接ELISA试验对口蹄疫具有较好的特异性，可以区分感染口蹄疫病毒的猪血清和疫苗免疫猪血清。
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| 关 键 词： | 口蹄疫 3A蛋白 ELISA 间接ELISA 猪口蹄疫 病毒感染 试验检测 A蛋白 ELISA试验 口蹄疫病毒 |
| 文章编号： | 1005-944X(2005)11-0020-03 |
Studies on Application of Indirect 3A Protein-ELISA for Detection of FMD Antibodies in Swines |
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| Gong ZhenHu,Jiang ZhengJun,Wang RuoCong,Hu YongHao,Liu JunHui,Chon ShuKun,Dong ZiZhen,Qi HuiCai,Guang YuanHong,Wang YuDong,Zheng ZengRen.Studies on Application of Indirect 3A Protein-ELISA for Detection of FMD Antibodies in Swines[J].China Journal Of Animal Quarantine,2005,22(11):20-22. |
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| Authors: | Gong ZhenHu Jiang ZhengJun Wang RuoCong Hu YongHao Liu JunHui Chon ShuKun Dong ZiZhen Qi HuiCai Guang YuanHong Wang YuDong Zheng ZengRen |
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| Institution: | Natimal Animal Quarantine Institute,MOA, Qingdao 266032,China |
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| Abstract: | An indirect ELISA was established with expressed 3A protein of was experimentally used to detect FMDV infection sera,immune sera and other the indirect 3A-ELISA was specific to FMDV infection and can distinquish FMD immune sera. FMDV as coating antigen and disease sera. It was shown that FMDV infection sera from the |
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| Keywords: | Foot and mouth disense 3A protein ELISA |
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