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养殖河豚鱼弧菌病原菌的鉴定和生物学特性分析
引用本文:麻丽丹,王殿夫,于晓婕,吴斌.养殖河豚鱼弧菌病原菌的鉴定和生物学特性分析[J].中国动物检疫,2011,28(7):40-42.
作者姓名:麻丽丹  王殿夫  于晓婕  吴斌
作者单位:1. 丹东出入境检验检疫局,辽宁丹东,118000
2. 辽东学院动物科学系,辽宁丹东,118000
3. 四川大学生命科学学院,四川成都,610065
4. 辽宁出入境检验检疫局,辽宁大连,116001
基金项目:辽宁出入境检验检疫局科技项目,国家质检总局课题
摘    要:从鳍基部、躯干部以及腹面出现斑点状出血的患病河豚鱼病灶处分离出11株优势菌(M1~M11),以体壁肌肉注射方式进行感染实验,证实菌株M2、M3~M6和M10为养殖河豚鱼鳍基部、躯干部以及腹面出现斑点状出血的病原菌,经形态学观察、生理生化特性分析和实时PCR检测,鉴定结果均为河弧菌。对15种药物的敏感性试验证实该菌株对氯霉素、环丙沙星和诺氟沙星较为敏感。

关 键 词:河豚鱼  皮下充血病  河弧菌  生物学特性  实时PCR

Identification and Biological Characterization of Vibrio Pathogen Associated with Cultured Swellfish
Ma Lidan,Wang Dianfu,Yu Xiaojie,Wu Bin.Identification and Biological Characterization of Vibrio Pathogen Associated with Cultured Swellfish[J].China Journal Of Animal Quarantine,2011,28(7):40-42.
Authors:Ma Lidan  Wang Dianfu  Yu Xiaojie  Wu Bin
Institution:Ma Lidan1,Wang Dianfu2,Yu Xiaojie3,Wu Bin4(1.Dandong Exit-Entry Inspection and Qurantine Bureau of the P.R.C,Dandong,Liaoning 118000,China,2.Department of Animal Science,Liaodong College,3.College of Life Science,Sichuan University,Sichuan 610065,4.Liaoning Exit-Entry Inspection and Qurentine Bureau of the P.R.C,Dalian,Liaoning 116001,China)
Abstract:Eleven bacterial strains named M1-Mll were isolated from the cultured swellfish with dermahemia in abdominal. pinna and truncal region.The strains M2,M3-M6 and M10 were proved to be pathogen by muscle injection.By meens of morphological observation, physiological and biochemical characteristic analysis and PCR detection, They were identified as Vibrio fluvialis.The results of sensitivity test with 15 antibacterial agents showed that they were sensitive to Chloramphenicol, Ciprofloxacin and Norfloxacin.
Keywords:swellfish  dermahemia of abdominal regiomV  fluvialis  biological characteristic  real-time PCR
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