| 光敏生物素标记EDSV-DNA探针检测鸡减蛋综合症(EDS)的研究 |
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| 引用本文： | 曹洪敬,徐学孟,王文志,杜玉芹,宫云浩,孙淑芳.光敏生物素标记EDSV-DNA探针检测鸡减蛋综合症(EDS)的研究[J].中国动物检疫,1994(1):7-8. |
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| 作者姓名： | 曹洪敬 徐学孟 王文志 杜玉芹 宫云浩 孙淑芳 |
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| 作者单位： | 山东省农科院畜牧兽医研究所!250010(曹洪敬，徐学孟，王文志，杜玉芹)，农业部动物检疫所!266032(宫云浩，孙淑芳) |
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| 摘 要： | 应用光敏生物素标记EDSV－DNA与pUC19重组质粒DNA做探针，检测人工发病减蛋综合症蛋鸡的粪便、蛋、输卵管样品，以新城疫病毒（NDV）、传染性囊病病毒（IBDV）、传染性支气管炎病毒（IBV）、包涵体肝炎病毒（IBHV）作对照。实验结果显示：探针能特异地检出EDS病鸡粪便、输卵管、蛋清样品中的病毒，与NDV、IBDV、IBV及IBHV均呈阴性反应。探针灵敏度达10pgEDSV-DNA。
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| 关 键 词： | 减蛋综合症 DNA探针 |
Study on Photobiotin-labelled DNA Probe for the Detection of Egg Drop Syndromes virus |
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| Cao Hong-jing, Xue-meng, Wang Wen-zhi, Du Yu-qing.Study on Photobiotin-labelled DNA Probe for the Detection of Egg Drop Syndromes virus[J].China Journal Of Animal Quarantine,1994(1):7-8. |
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| Authors: | Cao Hong-jing Xue-meng Wang Wen-zhi Du Yu-qing |
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| Abstract: | Samples of feces, egg and oviducts from egglayers infected experimentally with egg drop syndrome virus were examined by using photobiotin-labelled EDSV-DNA and pUC-19 plasma recombinant as probe. The results showed that the probe could specifically detect EDSV in the samples of feces, oviducts and whey from the infected birds and showed negative reactions to the controls as NDV, IBDV, IBV and IBHV. The sendtivity of the probe reached 10pg EDSV-DNA. |
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| Keywords: | Egg-drop-syndromes DNA probe |
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