| 猪细小病毒SH-1株VP2和NS1基因的克隆与原核表达 |
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| 引用本文： | 张春玲,张婉华,臧克伟,邹勇,李春华,蒋凤英,何锡忠,王英.猪细小病毒SH-1株VP2和NS1基因的克隆与原核表达[J].中国动物检疫,2007,24(9):23-25. |
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| 作者姓名： | 张春玲 张婉华 臧克伟 邹勇 李春华 蒋凤英 何锡忠 王英 |
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| 作者单位： | 1. 上海农科院畜牧兽医研究所,201106
2. 山东农业大学,山东泰安,271018 |
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| 摘 要： | 通过PCR技术,从感染了猪细小病毒的ST细胞中扩增出猪细小病毒的VP2基因和NS1基因,并将其亚克隆至原核表达载体中,分别构建了重组表达载体pET32c/VP2和pET30a/NS1,经IPTG诱导,使pET32c/VP2和pET30a/NS1在受体菌BL21(DE3)中得到高效表达。经Western-blot检测具有生物学活性,为猪细小病毒野毒与疫苗毒的鉴别诊断奠定基础。
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| 关 键 词： | 猪细小病毒 表达 |
| 文章编号： | 1005-944X（2007）09-0023-03 |
Molecular Cloning and Prokaryotic Expression of VP2 and NS1 Gene of Porcine Parvovirus SH Strain |
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| ZHANG chunling,ZHANG wanhu,ZANG kewei,ZOU yong,LI chuhu,JIANG fengying,HE xizhong,WANG ying.Molecular Cloning and Prokaryotic Expression of VP2 and NS1 Gene of Porcine Parvovirus SH Strain[J].China Journal Of Animal Quarantine,2007,24(9):23-25. |
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| Authors: | ZHANG chunling ZHANG wanhu ZANG kewei ZOU yong LI chuhu JIANG fengying HE xizhong WANG ying |
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| Institution: | 1.Animal Husbandry and Veterinary Researy Institute, shanghai Academy of Agricultural Sciences, Shanghai 201106; 2.College of Veterinary Medicine,shangdong Agricultural University, Taian 271018 |
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| Abstract: | In this study , the open reading frame of PPV VP2 and NS1 were amplified by PCR method from the PPV infected ST cells. Then the products were cloned in prokaryotic vector and pET32c/VP2 and pET30a/NS1expressed were construed respectively .The plasmids were introduced into E. coli BL21. After induction by IPTG , the high expression were found in products of pET32c/VP2 and pET30a/NS1 . Biological activity of the recombinant protein were detected by Western-blot , which can be applied in differential diagnosis of PPV infections. |
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| Keywords: | VP2 NS1 |
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