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| 检测流感病毒的商业PCR试剂盒比对验证 | |
| 引用本文： | 李 健,黄忠荣,王 权,姜平.检测流感病毒的商业PCR试剂盒比对验证[J].中国动物检疫,2011,28(11):32-34. |
| 作者姓名： | 李 健 黄忠荣 王 权 姜平 |
| 作者单位： | 1. 南京农业大学动物医学院,江苏南京,210095 2. 上海出入境检验检疫局,上海,200135 3. 中国农科院上海兽医研究所,上海,200241 |
| 基金项目： | 质检公益项目(200910132-04)“潜在入侵的高风险动物疫病口岸快速筛查技术研究” |
| 摘 要： | 利用8种不同流感病毒株及其核酸,结合自然感染组织样品与人工混合样,验证比较了8种商业PCR试剂盒的质量参数。结果表明,3号荧光PCR试剂盒漏检1株外,其他试剂盒均可以检测8种毒株,对其他类似病毒如猪繁殖与呼吸综合征病毒、猪伪狂犬病毒、猪传染性胃肠炎病毒、猪细小病毒和猪环状病毒核酸等无交叉反应,最高可检测到10-7稀释的目标核酸,重复性与再现性均为理想,在有效期内检测结果没有发生变化。 |
| 关 键 词： | 流感病毒 PCR 试剂盒 验证 |
Validation of Commercial RT-PCR and Real-time RT-PCR kits for Detection of Influenza Virus |
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| Li Jian,Huang Zhongrong,Wang Quan,Jiang Ping.Validation of Commercial RT-PCR and Real-time RT-PCR kits for Detection of Influenza Virus[J].China Journal Of Animal Quarantine,2011,28(11):32-34. | |
| Authors: | Li Jian Huang Zhongrong Wang Quan Jiang Ping |
| Institution: | Li Jian1,2,Huang Zhongrong2,Wang Quan3,Jiang Ping1 (1.College of veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China,2.Shanghai Entry-Exit Inspection and Quarantine Bureau,Shanghai 200135,3.Shanghai Veterinary Institute of CAAS,Shanghai 200241,China) |
| Abstract: | Five commercial real-time RT-PCR kits and three RT-PCR kits were assessed with eight influenza viruses.The different criteria assessed were sensitivity,specificity,reproducibility using different samples.These samples were inactivated strains or organs naturally infected or Spiked biological samples.The results proved these kits to be sensitive with high specificity and reproducibility. |
| Keywords: | Influenza virus PCR kits validation |
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