|
|||||
|
|
| 一株狂犬病病毒街毒株的分离鉴定与组织培养 | |
| 引用本文： | 徐振波,潘铁骊,张守峰,张洋,徐慧娟,王燕,扈荣良.一株狂犬病病毒街毒株的分离鉴定与组织培养[J].中国动物检疫,2008,25(9):22-24. |
| 作者姓名： | 徐振波 潘铁骊 张守峰 张洋 徐慧娟 王燕 扈荣良 |
| 作者单位： | 1. 吉林大学畜牧兽医学院,吉林长春,130062;军事医学科学院军事兽医研究所,吉林长春,130062 2. 军事医学科学院军事兽医研究所,吉林长春,130062 3. 青岛农业大学动物科技学院,山东青岛,266109 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 对来自河北省无极县某地区疑似狂犬病病犬脑组织进行FAT、电镜观察、RT-PCR扩增,以及MIT法等确诊为狂犬病,对分离到的这株狂犬病街毒命名为WJ07-1,分析N蛋白和G蛋白序列,主要抗原位点发生轻度变异,同源性分析发现N基因与ZhejiangWz(H)株同源性最高为99.0%,G基因与JSL27株同源性最高为99.4%。对分离到的狂犬病病毒进行组织培养发现这株狂犬病病毒街毒株对细胞的适应性和感染性很弱,需要进行多次传代培养,才可以适应N2a、BHK-21细胞。 |
| 关 键 词： | 狂犬病病毒 RT-PCR FAT MIT 组织培养 |
Isolation, Identification and Tissue Culture of a Street (Rabies) Virus Strain |
|
| Xu Zhenbo,Pan Tieli,Zhang Shoufeng,Zhang Yang,Xu Huijuan,Wang Yan,Hu Rongliang.Isolation, Identification and Tissue Culture of a Street (Rabies) Virus Strain[J].China Journal Of Animal Quarantine,2008,25(9):22-24. | |
| Authors: | Xu Zhenbo Pan Tieli Zhang Shoufeng Zhang Yang Xu Huijuan Wang Yan Hu Rongliang |
| Institution: | 1.College of Animal Science and Veterinary Medicine, Jilin University,Changchun 130062,china; 2.Military Veteriary Institute,Academy of Military Medical Sciences, Changchun 130062,China; 3.College of Veteriary and Animal Sci- ence,Qingdao Agricultural University,Qingdao 266109,China) |
| Abstract: | FAT, electron microscope, RT-PCR,and MIT were used to diagnose a street (rabies) virus strain from Wuji County Hebei province,which was named WJ07-1. Few variation of the major antigen sites were found after analysing the N gene and G gene sequences, homology of nucleotide of N gene with ZhejiangWz (H) was 99.0%; homology of nucleotide of G gene with JSL27 was 99.4%. Adaptability and infectivity on cells were found weak af- ter tissue culture,More subcultures are needed for it to adapt N2a cells and BHK-21 cells. |
| Keywords: | rabies virus RT-PCR FAT MIT tissue culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国动物检疫》浏览原始摘要信息 | |
| 点击此处可从《中国动物检疫》下载免费的PDF全文 | |