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| 应用间接ELISA检测猪旋毛虫抗体 | |
| 引用本文： | 何光志,田维毅,王平,王文佳,韩洁,简昌友,安永如.应用间接ELISA检测猪旋毛虫抗体[J].中国动物检疫,2010,27(1):47-48. |
| 作者姓名： | 何光志 田维毅 王平 王文佳 韩洁 简昌友 安永如 |
| 作者单位： | 1. 贵阳中医学院,贵州贵阳,550002 2. 凤冈县人民医院,贵州凤冈,564200 3. 安顺市职业技术学院,贵州安顺,561000 |
| 摘 要： | 以猪旋毛虫抗原基因Ts88的重组蛋白为包被抗原,建立了猪旋毛虫抗体间接ELISA检测方法。最佳抗原包被浓度为1μg/mL,待检血清的最佳稀释倍数为1:80。采用间接ELISA方法检测2000份猪血清,阳性检出率为1.5%,血清样本对应猪肉采用镜检法,阳性检出率为1.30%。试验结果显示,该方法操作简便、快速、特异性好,适用于猪旋毛虫的临床诊断和流行病学调查。 |
| 关 键 词： | 猪旋毛虫 间接ELISA 猪旋毛虫抗体 |
Development of an Indirect Enzyme-Linked Immunosorbent Assay(I-ELISA)for Trichinosis |
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| He Guangzhi,Tian weiyi,Wang Ping,Wang Wenji,Han Jie,Jian Changyou,An Yongru.Development of an Indirect Enzyme-Linked Immunosorbent Assay(I-ELISA)for Trichinosis[J].China Journal Of Animal Quarantine,2010,27(1):47-48. | |
| Authors: | He Guangzhi Tian weiyi Wang Ping Wang Wenji Han Jie Jian Changyou An Yongru |
| Institution: | 1, Guiyang college of Traditional Chinese Medicine, Guizhou Guiyang 550002; 2, Fenggang County People’s Hos- pital, Guizhou, Fenggang 564200; 3, Anshun Vocational and Technical College,Guizhou Anshun 561000) |
| Abstract: | Based on the purified the recombinant Ts88 proteins, a indirect ELISA (I-ELISA) was developed to detect Trichinella spiralis anti-body in pigs. For the indirect ELISA method, the optimal concentration of coating antigen in I-ELISA was 1μg/mL, the optimal dilution of the serum was 1:80. The positive rate was 1.5 percent from 2000 serum samples by I-ELISA, whereas the microscope test was 1.30 percent. The present study suggested that the method is very simple,specific and rapid,and very useful for clinical diagnosis and epidemiological survey of Trichinosis. |
| Keywords: | Trichinella spiralis ELISA Serum antibody to Trichinella spiralis |
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