| 肠艾美尔球虫纯种分离及18S rDNA部分序列测定 |
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| 引用本文： | 崔平,顾小龙,方素芳,刘红彬.肠艾美尔球虫纯种分离及18S rDNA部分序列测定[J].中国动物检疫,2010,27(8):40-43. |
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| 作者姓名： | 崔平 顾小龙 方素芳 刘红彬 |
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| 作者单位： | 河北北方学院动科院,河北,张家口,075000 |
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| 基金项目： | 国家兔产业技术体系(农业部)资助项目 |
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| 摘 要： | 采用单卵囊分离技术,分离肠艾美尔球虫。根据GenBank中发表的肠艾美尔球虫18SrDNA序列,设计一对引物,建立PCR方法对其基因片段扩增并测序、比对。结果成功分离肠艾美尔球虫,PCR扩增出清晰条带,大小为528bp,最低能检出27个孢子化卵囊。该序列测定结果与Genebank发表的肠艾美尔球虫18SrDNA比对,相似性达98.5%。
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| 关 键 词： | 肠艾美尔球虫 单卵囊分离 18SrDNA 测序 |
The Pure Specie Isolation and 18S rDNA Part Sequence of Eimeria Intestinalis |
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| Cui Ping,Gu Xiaolong,Fang Sufang,Liu Hongbin.The Pure Specie Isolation and 18S rDNA Part Sequence of Eimeria Intestinalis[J].China Journal Of Animal Quarantine,2010,27(8):40-43. |
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| Authors: | Cui Ping Gu Xiaolong Fang Sufang Liu Hongbin |
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| Institution: | (Colloge of Animal Science and Technology,Hebei North University Zhangjiakou 075000,China) |
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| Abstract: | The pure strain Eimeria intestinalis was isolated by single oocyst separation technology,a pair of primer was designed according to the published 18S rDNA sequence of E.intestinalis in the GenBank,and a PCR method was established to amplify the gene fragment for sequencing and comparison.Results indicated that pure strain of E.intestinalis was isolated and its gene fragment was amplified with 528 bp in length.The PCR method could detect 27 sporulated oocysts.Similarity of gene sequences was up to 98.5% between the previously published 18S rDNA sequence of E.intestinalis and the amplified 528bp sequence by comparison. |
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| Keywords: | 18S rDNA |
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