分子生物技术在牛奶微生物群落研究中的应用

目前,奶牛乳房炎病原学研究的主要方法为对牛奶中的微生物进行传统的分离鉴定。由于这些微生物的形态过于简单,可提供的信息少,而且很多无法进行纯培养,传统方法已无法满足对其群落进行全面的客观认识。随着现代分子生物学技术的发展,随机扩增多态性DNA标记、变性梯度凝胶电泳、单链构象多态性检测、实时荧光定量PCR、核酸探针等技术为牛奶中微生物群落的研究提供了更为有效、直观的方法,更进一步揭示了牛奶中微生物的种群结构。

Application of Molecular Biology Techniques to Study Microbial Community in Milk

Currently for dairy cow mastitis pathogen research mainly by microorganisms in milk were the traditional isolation and identification, due to microbial morphology is too simple, cannot provide too much information, and many microorganisms cannot be cultured in vitro, it gives an objective understanding of milk in the microorganisms present condition cause serious obstacle. The development of modem molecular biology technique for the study of microorganisms in milk provides a more effective and intuitive method, using random amplified polymorphic DNA markers, denaturing gradient gel electrophoresis, single-strand conformation polymorphism detection technology, real time fluorescent quantitative PCR technique, technique of nucleic acid probes on microorganisms in milk for further study and research, a more comprehensive reveals population structure of microorganisms in milk.