牛病毒性腹泻病毒弱毒活疫苗免疫持续期的研究

为检测牛病毒性腹泻病毒(BVDV)弱毒活疫苗在免疫牛体内抗体产生及其消长规律,评价弱毒疫苗的保护效力,并确定免疫持续期,本试验对免疫试验牛每头颈部肌肉接种BVDV SM株弱毒疫苗104.5TCID50,监测血清抗体效价,进行免疫持续期的确定。在疫苗免疫后的6个月、9个月和12个月,分别抽取5头免疫组和5头对照组牛,采用BVDV-JL强毒株进行攻毒试验,每头牛攻毒剂量为6×107.0TCID50/mL。结果显示疫苗免疫后12个月时血清中和抗体效价仍维持在1∶1048以上。攻毒结果显示,在3个不同时间点进行强毒攻击后,免疫组所有动物白细胞数量都没有下降也没有分离到病毒,而对照组动物白细胞数下降均超过30%,6个月和9个月时动物血清中均能分离到病毒,而12个月对照组动物由于年龄大,没有分离到病毒,因此暂定此疫苗的免疫持续期为9个月。

The Efficacy of the Live Attenuated Bovine Viral Diarrhea Virus SM Vaccine Strain

The study was designed to evalutate neutralizing（SN） antibodies against bovine viral diarrhea virus（BVDV） in the calves vaccinated with the live attenuated bovine viral diarrhea virus SM vaccine.A total of 30 healthy calves at weaning were randomly divided into two groups,with 15 calves in vaccinate group and 15 in control group.The attenuated BVDV SM vaccine strain was administrated via intramuscular injection on the neck at a single dose and challenged with a virulent BVDV-JL virus by nasal spray.The blood sample were collected at different time points for monitoring SN antibody titers.The results indicated that the SN antibody titers in the vaccinated calves maintained at a relative high level until 12 month post-vaccination,and the vaccinated calves were effectively protected from the challenge,as detected by the white blood cell count（WBC） and,virus shedding.therefore,the duration of immunity of the vaccine was at lease for 9 months.