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应用斑点酶联免疫吸附试验快速检测饲料中的沙门氏菌
引用本文:雷风,韩志辉,张红见. 应用斑点酶联免疫吸附试验快速检测饲料中的沙门氏菌[J]. 中国奶牛, 2005, 0(5): 9-11
作者姓名:雷风  韩志辉  张红见
作者单位:青海大学农牧学院,西宁,810003
摘    要:选用硝酸纤维素(NC)膜作固相载体,建立检测沙门氏菌的斑点酶联免疫吸附试验诊断方法,检测成品饲料100份,对沙门氏菌的最低检出量为400个/mL,沙门氏菌阳性检出率43.00%(43/100),常规分离培养阳性检出率为38.00%(38/100),两者符合率为83.72%,差异不显著(P>0.05)。试验表明该方法简便、特异、快速、结果直观,便于在基层推广使用。

关 键 词:斑点酶联免疫吸附试验 检测 成品饲料 沙门氏菌
文章编号:1004-4264(2005)05-0009-03
收稿时间:2004-10-09
修稿时间:2004-10-09

Checking Salmonella in Animal Feed by Dot-ELISA
Lei Feng,Han Zhihui,Zhang Hongjian. Checking Salmonella in Animal Feed by Dot-ELISA[J]. China Dairy Cattle, 2005, 0(5): 9-11
Authors:Lei Feng  Han Zhihui  Zhang Hongjian
Abstract:A Dot-enzyme linked immunosorbentassay was successfully developed by using nitrocellulose membrane as solid carrier,100 anim alfeeds were checked,the results showed thatthe m inim um was 400 salm onella/m L,and the positive rate ofsalm onella was 43.00%(43/ 100).the positive rate was 38.00%(38/100) identified by the technology of routine isolation and culture.positive coincidence rate of both m ethods was 83.72% and there was no significantdifference(P>0.05).the trialindicated thatthis m ethod was sim ple,special,quick,directand easy extention and application atprim ary level.
Keywords:Dot-ELISA   Checking   Animal feed   Salmonella
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