| 抗IVM多克隆抗体检测牛组织中IVM残留的研究 |
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| 引用本文： | 魏小娟,张继瑜,张梅,李剑勇,周绪正,李金善.抗IVM多克隆抗体检测牛组织中IVM残留的研究[J].中国奶牛,2008(4):14-16. |
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| 作者姓名： | 魏小娟 张继瑜 张梅 李剑勇 周绪正 李金善 |
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| 作者单位： | 中国农业科学院兰州畜牧与兽药研究所,兰州,730050 |
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| 摘 要： | 用自制的抗伊维茵素（IVM）多克隆抗体检测牛肌肉和肝脏中IVM的残留，将IVM以5．0ng／g、10．0ng，g、20．0ng／g和40．0ng／g的量分别添加至经处理的各空白组织中，用竞争ELISA法测得IVM在肌肉和肝脏中的回收率为93．9％～98．3％，变异系数为2．1％～5．1％，最低检测限为0．94ng／g。
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| 关 键 词： | 伊维菌素 牛组织 竞争ELISA 残留 |
| 文章编号： | 1004-4264(2008)04-0014-02 |
| 修稿时间： | 2008年1月2日 |
Study on the Detection of Residue of Ivermectin in Bovine Tissues with Anti-ivermectin Polyclonal Antibody |
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| Wei Xiaojuan,Zhang Jiyu,Zhang Mei,Li Jianyong,Zhou Xuzheng,Li Jinshan.Study on the Detection of Residue of Ivermectin in Bovine Tissues with Anti-ivermectin Polyclonal Antibody[J].China Dairy Cattle,2008(4):14-16. |
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| Authors: | Wei Xiaojuan Zhang Jiyu Zhang Mei Li Jianyong Zhou Xuzheng Li Jinshan |
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| Institution: | Wei Xiaojuan, Zhang Jiyu, Zhang Mei, IA Jianyong, Zhou Xuzheng, Li Jinshan (Lanzhou Institute of Animal and Veterinary Pharmaceutics Sciences, Lanzhou 730050) |
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| Abstract: | We detected the residue of Ivermectin in bovine＇s liver and muscle tissues by homemade anti-Ivermectin polyclonal antibody.We added Ivermectin to bovine tissues at levels of 5.0ng/g, 10.0ng/g,20.0ng/g and 40.0ng/g respectively and the recoveries was 93.9%～98.3% by competitive ELISA.Variation coeficient of the assay was 2.1%～5.1%,the detection limit was 0.94ng/g. |
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| Keywords: | Ivermectin Bovine tissues Competitive ELISA Residue |
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