PCR和斑点杂交检测鸡传染性贫血病毒

通过聚合酶链反应（ｐｃＲ）扩增鸡传染性贫血病毒（ｃＡＶ）ＤＮＡ特定片段，将此ｐｃＲ产物用Ｄｉｇｏｘｉｇｅｎｉｎ标记后作为探针进行斑点杂交，以此检测ＣＡＶ并作流行病学调查。对从江苏省不同地市随机采集的２月龄左右不同疾病的病鸡ＤＮＡ样品进行检测，在被检的５２个不同鸡群来源的病料中，有３６个鸡群来源的病料ＤＮＡ显示ＣＡＶ阳性（群阳性率为６９．２％）；备组织中ＣＡＶＤＮＡ含量有所差异，依次为胸腺＞脾、骨髓、肝＞肾、法氏囊、脑。用ＰＣＲ检测得到的阳性鸡的组织病料感染ＳＰＦ鸡，在感染后第２４天检查，出现贫血症状。初步调查表明，在江苏一些地区ＣＡＶ感染已相当普遍，但它与发病的关系还有待于进一步研究。

Use of Dot Blot Hybridization and PCR to Detect ChickenAnaemia Virus

The special fragments of the chicken anaemia virus(CAV) DNA wereamplified by the polymerase chain reaction(PCR).The CAV virus was detected and theepidemiology was surveyed by dot blot hybridization assay using the PCR products la-belled with Digoxigenin as probes.Using this probe,the tissue DNA samples of differ-ent ill chickens about two-month-old collected randomly from different areas of Jiangsuprovince were detected by the dot blot hybridization assay,36 out of 52 chicken flockswere detected positive(the positive rate was 69.2 percent).The CAV DNA abundancesin different tissues were not same,i.e.thymus > spleen,bone marrow,liver>kidney,bursa of Fabricius,brain,The SPF chickens showed anaemia on the 24th day after inoc-ulation of the PCR positive tissue sample.The preliminary investigation showed that theCAV infection is very prevalent in many areas of Jiangsu province,but the relation be-tween infection and illness needs to be studied further.