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| 牛肌肉生长抑制素基因单核苷酸多态性分析 | |
| 引用本文： | 岳续朋,戴立胜,赵锐锋,李喜春,赵志辉,张嘉保.牛肌肉生长抑制素基因单核苷酸多态性分析[J].中国兽医学报,2009,29(10). |
| 作者姓名： | 岳续朋 戴立胜 赵锐锋 李喜春 赵志辉 张嘉保 |
| 作者单位： | 1. 吉林大学,实验动物中心,吉林,长春,130062;吉林大学,畜牧兽医学院,吉林,长春,130062 2. 吉林省长春优质肉牛繁育中,吉林,长春,130062 3. 吉林大学,畜牧兽医学院,吉林,长春,130062 4. 吉林大学,实验动物中心,吉林,长春,130062 |
| 基金项目： | 国家科技支撑计划，吉林省科技发展重点项目 |
| 摘 要： | 应用PCR-SSCP分析方法,对94头肉牛(公牛45头:西门塔尔34头,夏洛来11头;母牛49头:西门塔尔24头,夏洛来25头)的肌肉生长抑制素(MSTN)3个外显子进行了多态性分析.结果显示,第1外显子存在2种基因型,分别为AA型和AB型.经测序发现,第1外显子4 bp处存在C→G的碱基突变,导致编码的氨基酸由谷氨酰胺(Gln)→谷氨酸(Glu).统计结果表明,等位基因B的含量低,而且只在西门塔尔品种内含0.026 6.利用SPSS软件作最小二乘分析,结果表明,B等位基因与西门塔尔牛的成年体质量和犊牛出生体质量呈显著正相关. |
| 关 键 词： | 肌肉生长抑制素 单核苷酸多态性 牛 |
Single nucleotide polymorphism analysis of myostatin gene in cattle |
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| YUE Xu-peng,DAI Li-sheng,ZHAO Rui-feng,LI Xi-chun,ZHAO Zhi-hui,ZHANG Jia-bao.Single nucleotide polymorphism analysis of myostatin gene in cattle[J].Chinese Journal of Veterinary Science,2009,29(10). | |
| Authors: | YUE Xu-peng DAI Li-sheng ZHAO Rui-feng LI Xi-chun ZHAO Zhi-hui ZHANG Jia-bao |
| Abstract: | By PCR-SSCP approach,single nucleotide polymorphisms(SNPs) of cattle myostatin gene(MSTN) were analyzed in Simmental cattle and Charolais cattle.There were two genetypes in the first exon,AA and AB.A transversion at nucleotide position 4 of the myostation in the first exon led to a conservative Glutamine-to-Glutamic substitution at amino acid position 2.The statistics indicated that the content of Allele B was low.The content of Allele B in Simmental cattle and Charolais cattle was 0.026 6 and 0.00.By SPSS software calculate least squares means and std err,the allele B has a positive correlation with birth weight and adult weight of Simmental cattle. |
| Keywords: | myostatin single nucleotide polymorphism cattle |
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