| 牛结核分枝杆菌溶血磷脂酶真核表达载体的构建及在Hela细胞中的表达 |
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| 引用本文： | 贾浩,徐广贤,周萍,张艳,王玉炯.牛结核分枝杆菌溶血磷脂酶真核表达载体的构建及在Hela细胞中的表达[J].中国兽医学报,2010,30(2). |
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| 作者姓名： | 贾浩 徐广贤 周萍 张艳 王玉炯 |
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| 作者单位： | 宁夏大学,生命科学学院,西部特色生物资源保护与利用教育部重点实验室,宁夏,银川,750021 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，国家自然科学基金，高等学校科技创新工程重大项目培育资金，宁夏高校科研资助项目 |
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| 摘 要： | 为了研究牛结核分枝杆菌溶血磷脂酶基因(Lip)的生物学特性及其功能,试验构建了Lip-pEGFP融合基因的真核表达载体,利用脂质体(lipofectamine2000)将其转染到Hela细胞中,用荧光显微镜检测GFP在细胞中的表达情况。结果显示,重组Lip-pEGFP载体在Hela细胞中获得了高效表达,且能够稳定传代,为进一步研究Lip的生物学功能及其在牛结核分枝杆菌中的作用奠定了基础。
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| 关 键 词： | 牛结核分枝杆菌 溶血磷脂酶 Hela细胞 |
Construction of eukaryotic expression vector Lip-pEGFP and its expression in Hela cells |
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| JIA Hao,XU Guang-xian,ZHOU Pin,ZHANG Yan,WANG Yu-jiong.Construction of eukaryotic expression vector Lip-pEGFP and its expression in Hela cells[J].Chinese Journal of Veterinary Science,2010,30(2). |
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| Authors: | JIA Hao XU Guang-xian ZHOU Pin ZHANG Yan WANG Yu-jiong |
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| Abstract: | In order to study the biological character and functions of the gene encoding lysophospholipase (Lip)of Mycobacterium boris,the eukaryotic expression vector of fusion gene Lip-pEGFP was constructed. The vector was transfected into Hela cells by lipofectamine2000 and the expressed GFP protein was detected by fluorescence micro-scope. The results showed that the recombinant vector of Lip-pEGFP was highly expressed in Hela ceils and can be stable expressed and passaged. This laid a foundation for further study on the biological functions of Lip and its effect on Mycobacterium boris. |
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| Keywords: | Mycobacterium tuberculosis lysophospholipa Hela cells |
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