鸡传染性支气管炎病毒广西分离株血清型的分析

应用病毒感染的鸡胚材料免疫新西兰兔的方法制备抗鸡传染性支气管炎病毒(IBV)单因子血清,然后在鸡胚气管环培养(Tracheal organ cultures,TOC)上对广西分离的7个IBV代表性毒株和3个常用疫苗株进行交叉病毒中和试验。结果显示,10个毒株被分为6个血清型。根据试验所得的R值,应用聚类分析法分析了各血清型毒株之间的亲缘关系,显示目前在广西流行的IBV野毒株之间以及其与疫苗株间的抗原性存在很大程度的差异,分属不同的血清型。同时还对IBV基因分型和血清分型之间的关系进行了探讨。

Serotyping of Guangxi isolates of infectious bronchitis virus

Monovalent antisera against seven field isolates and three commonly used vaccine strains of infectious bron-chitis virus (IBV) were prepared in rabbits and were used in the virus neutralization test against the heterogeneous and homogeneous viruses on tracheal organ cultures (TOC) of chicken embryo. Relatedness values of each virus cal-culated from cross-neutralization antibody titer showed that 6 serotypes were distinguished among the 10 viruses tested. Phylogenetic analysis of the different serotypes of viruses also indicated that there was a divergence among the field isolates and between the isolates and the vaccine strains, belonging to different serotypes. The data of the study provides a reliable basis for catching up the emerged antigenic variation of the field viruses and developing more effective vaccine for the disease control in Guangxi. The relationship between genotyping and serotyping was al-so discussed.