新型鸭肝炎病毒分离株JFX08全基因组测定与分析

对我国鸭病毒性肝炎病毒(Duck hepatitis virus,DHV)分离株JFX08的全基因组进行了序列测定与分析。结果表明,JFX08毒株的基因组全长为7793nt,包括652nt的5’UTR,6753nt的ORF,366nt的3’UTR以及19nt的poly(A)尾巴。JFX08毒株的ORF编码2251个氨基酸,各基因均与韩国新型毒株的氨基酸序列相似性最高,与1型DHV和台湾新型DHV的序列相似性均较低。其中VP1较1型DHV存在2个氨基酸的插入和较多氨基酸位点的突变,高变区主要集中在180～194位和213～219位。JFX08毒株的非编码区核苷酸序列之间存在大量碱基突变和插入/缺失。多聚蛋白、VP0、VP3、VP1、2C和3D基因进化分析结果均表明,JFX08分离株与韩国新型DHV的遗传距离最近,属基因C型。

Sequencing and analysis of genome of duck hepatitis virus isolated from China

The genome of duck hepatitis virus strain JFX08 isolated from China has been sequenced and analyzed. The results showed that the full length of the genome was 7 793 nt,including 652 nt of 5'untranslated region (UTR) ,an open reading frame of 6 753 nt encoded a polypeptide of 2 251 amino acids, 366 nt of 3' untranslated region. The pairwise percent identity of the amino acid sequences at ORF region between strain JFX08 and Korea new type DHV were higher than that of between strain JFX08 and 1 type DHV or Taiwan new type DHV. There were 2 amino acids insertion and many variant sites and the hypervariable regions were found in 180-194 aa and 213-219 aa of VP1 gene. A lot of variant sites and insertion/deletion were found in untranslated region. Phylogenetic and evolutionary analy-sis based on polyprotein,VP0,VP3,VP1,2C and 3D indicated that strain JFX08 had a very close relationship with Korea new type DHV,which affiliated with genotype C.