| 用PCR检测副溶血性弧菌耐热溶血素基因 |
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| 引用本文： | 周志江,刘纯杰,黄上媛,中野克重,佐藤久聪,齐藤博.用PCR检测副溶血性弧菌耐热溶血素基因[J].中国兽医学报,1996(3). |
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| 作者姓名： | 周志江 刘纯杰 黄上媛 中野克重 佐藤久聪 齐藤博 |
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| 作者单位： | 解放军农牧大学兽医学院，日本国北里大学兽医畜产学部，解放军３０２医院微生物研究室 |
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| 摘 要： | 根据副溶血性弧菌的耐热溶血素基因（ｔｄｈ）设计了２对引物，经聚合酶链式反应（ＰＣＲ）检测，所有１８株神奈川试验阳性副溶血性弧菌均呈阳性反应，而６株神奈川试验阴性副溶血性弧菌呈阴性反应，其他９种（３４株）弧菌和８株其他革兰氏阴性菌也呈阴性反应。检测敏感性可达２ＣＦＵ的副溶血性弧菌。
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| 关 键 词： | PCR，副溶血性弧菌，tdh基因 |
Detection of the Thermostable Direct Hemolysin (tdh) Gene of Vibrio parahaemolyticus by
Polymerase Chain Reaction |
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| Zhou Zhijiang,Liu Chunjie,Huang Shangyuan.Detection of the Thermostable Direct Hemolysin (tdh) Gene of Vibrio parahaemolyticus by
Polymerase Chain Reaction[J].Chinese Journal of Veterinary Science,1996(3). |
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| Authors: | Zhou Zhijiang Liu Chunjie Huang Shangyuan |
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| Abstract: | Two sets of primers based on the unique
regions of the tdh geiie of V.parahaemolyticus were designed.A specificity test dernonstrated
that the polymerasechain reaction (PCR) was positive for all 18 strains of Kanagawa
phenomenon (KP)-Positive V. parahaemolyticus but negative for 6 strains of KP-negative
V.para-haemolyticus and the other 9 species (34 strains)of Genus Vibrio,and negative for
8strains of the other gram-negative bacteria. This PCR method could detected as few as 2CFU
of V.parahaemolyticus. |
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| Keywords: | PCR Vibrio parahaemolyticus tdh gene Department of Microbiology The 302th Haspital of PLA |
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