种鸡接种网状内皮组织增生病病毒弱毒疫苗的安全性和免疫效果观察

在正常饲养条件下，在肉种鸡鸡群中试用网状内皮增生病病毒（reticuloendotheliosis virus，REV）的弱毒疫苗，观察其对体重增长、产蛋生产性能、对其他疫苗应答有无影响。同时连续定期测定种鸡血清REV抗体，并测试抗体阳性鸡的后代有无病毒垂直传播。结果表明，该疫苗接种18周龄种鸡后，对生长、产蛋率、受精率和孵化率等生产性能均无不良影响，对正常疫苗免疫的抗体应答也无影响。经免疫接种REV弱毒疫苗的种鸡，在开产后及产蛋高峰期，均不表现病毒的垂直传播。免疫种鸡后，其激发的抗体可持续280d以上，且雏鸡血清中母源抗体可持续至少7d。结果表明，该REV弱毒在开产前种鸡应用时有很高的安全性，并能为雏鸡提供足够的特异性母源抗体。

Observations of safety and antibody response in meat-type parent breeders inoculated with an attenuated reticuloendotheliosis virus

A group of meat-type parent breeders in a commercial farm was vaccinated with an attenuated reticuloendotheliosis virus (REV), and its influence on growth, egg production and immune reactions to other inactivated vaccines was studied. At the same time, antibody to REV in chicken sera at different intervals after vaccination was measured and vertical transmission of the virus was checked in chicks from vaccinated breeders. The results indicated that vaccination of 18-week-old parent breeders with the attenuated REV did not give any negative influence on growth rate, egg-productivity, egg fertility and hatchability. Antibody titers against Newcastle disease virus (NDV) and avian influenza virus (AIV) was also not affected after re-vaccination with inactivated vaccines to NDV, AIV-H5 or H9 in REV vaccinated birds as compared to the control. No vertical transmission of REV was detected in eggs or their chicks during the early period of laying or at the peak for egg production from REV-vaccinated breeders. REV-antibody in breeder sera could last at least for 280 days post vaccination. And the maternal antibody to REV provided by vaccination of breeders lasted at least for 7 days.