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应用ELISA(双抗体夹心法)检测猪流行性腹泻病猪粪便中的病毒抗原
引用本文:朱维正,王新平,常建兵.应用ELISA(双抗体夹心法)检测猪流行性腹泻病猪粪便中的病毒抗原[J].中国兽医学报,1988(2).
作者姓名:朱维正  王新平  常建兵
作者单位:兽医大学传染病教研室 (朱维正,王新平),兽医大学传染病教研室(常建兵)
摘    要:应用猪流行性腹泻(PED)—ELISA直接法(双抗体夹心法)对120头健康猪和5头猪传染性胃肠炎(TGE)病猪粪便标本进行检测,均不出现交叉反应;对15头PED病毒实验感染仔猪粪便标本检测,全部呈阳性;将对3(?)份ELISA阳性粪便标本和3份阴性标本的检测结果与电镜观察结果比较,其阳性符合率为97.37%,阴性符合率为100%;对在PED发病季节从不同地区采集的腹泻病猪粪便标本112份检测结果,阳性率为60.71%,而阴性反应的标本,绝大多数是在病愈后15~20d采集的。

关 键 词:ELISA(双抗体夹心法)  猪流行性腹泻病毒  抗原  粪便  

Detection of Virus Antigens in Feces of PED-infected Pigs with ELISA (Double Antibody Sandwich Method)
Zhu Weizhen et al..Detection of Virus Antigens in Feces of PED-infected Pigs with ELISA (Double Antibody Sandwich Method)[J].Chinese Journal of Veterinary Science,1988(2).
Authors:Zhu Weizhen
Abstract:A direct enzyme-linked immunosorbent assay for detecting PED virus antigens in feces of PED-infected pigs was developed. One hundred and twenty feces samples from healthy pigs and 5 of TGE-infected pigs revealed negative reactionos, but 15 fecal samples from experimental PED pigs were all positive. In order to compare ELISA and electron microsocopy for this purpose, 38 positive samples and 3 negative ones were examined simultaneously. The positive and negative rates were 97.37% and 100% in accordance with each other. One hundred and twelve samples collected from PED-infected herds in various regions were detected and the positive rate was 60.7%. Most of the samples taken at 15-20 day after recovery from PED showed negative results.
Keywords:ELISA (double antibody sandwich method)  PEDV  antigent feces  pig  
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