| 山羊免疫弓形虫代谢分泌抗原后的免疫应答 |
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| 引用本文： | 芦赟,张燕丽,曹丽艳,王艳华,苟惠天,付宝权,李文卉,张德林.山羊免疫弓形虫代谢分泌抗原后的免疫应答[J].中国兽医学报,2012,32(1):52-57. |
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| 作者姓名： | 芦赟 张燕丽 曹丽艳 王艳华 苟惠天 付宝权 李文卉 张德林 |
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| 作者单位： | 1. 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046／兰州市畜牧兽医研究所,甘肃兰州730050
2. 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州,730046 |
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| 基金项目： | 国家科技支撑计划资助项目(2007BAD40B05);公益性行业(农业)科研专项经费资助项目(200803017);甘肃省重大专项科技资助项目(2GS063-A43-013) |
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| 摘 要： | 将山羊随机分为6组，即E／SA纽、E／SA＋cpG组（未乳化）、E／SA＋CpG组、E／SA＋IL-2组、E／SA＋IL-2＋CpG组、对照组，每组3只，分别于免疫前、免疫后第2周、免疫后第4周、感染后第1周及感染后第2周采集山羊外周全血及涂血片，IHA法检测抗体滴度的动态变化；ELISA法检测IFN-γ、TNF—a、IL-2、IL-4的表达水平；ANAE染色法检测T淋巴细胞的动态变化。结果显示，免疫后各免疫组的IFN-γ、TNF-a、IL-2、IL-4及T淋巴细胞水平显著高于对照组（P〈0．05），各免疫组的抗体水平均高于对照组。结果表明，E／SA可引起IFN—γ、TNF—a、IL-2、IL-4、T淋巴细胞及抗体水平的升高，说明E／SA免疫后可引起山羊较强的细胞免疫和体液免疫应答。
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| 关 键 词： | 山羊 弓形虫代谢分泌抗原 免疫应答 |
Immune response of goat after immunized with Toxoplasma gondii excreted/secreted antigens |
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| LU Yun,ZHANG Yan-li,CAO Li-yan,WANG Yan-hua,GOU Hui-tian,FU Bao-quan,LI Wen-hui,ZHANG De-lin.Immune response of goat after immunized with Toxoplasma gondii excreted/secreted antigens[J].Chinese Journal of Veterinary Science,2012,32(1):52-57. |
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| Authors: | LU Yun ZHANG Yan-li CAO Li-yan WANG Yan-hua GOU Hui-tian FU Bao-quan LI Wen-hui ZHANG De-lin |
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| Institution: | 1*(1.Key Laboratory of Veterinary Parasitology of Gansu Province,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;2.Lanzhou Animal Husbandry and Veterinary Institute,Lanzhou 730050,China) |
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| Abstract: | To elucidate the immune mechanism of Toxoplasma gondii excreted/secreted antigens,goats were randomly divided into 6 groups,of E/SA,E/SA+CpG(without emuls ification),E/SA+CpG,E/SA+IL-2,E/SA+IL-2+CpG and the control group with 3 each.Peripheral blood were collected before immunization,on week 2,4 post-immunization,and on week 1,2 post-infection,respectively.The specific antibody titer was tested by IHA and the level of IFN-γ,TNF-α,IL-2 and IL-4 were analyzed by ELISA and the T cells were analyzed by ANAE.The results showed that the levels of IFN-γ,TNF-α,IL-2,IL-4,T cells and antibody in the 5 immune groups were significantly higher than in the control group(P<0.05).Research showed that E/SA could elevate the level of IFN-γ,TNF-α,IL-2,IL-4,T cells and antibody.Therefore,E/SA could result quickly in cellular immune response and the humoral immune response. |
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| Keywords: | goat Toxoplasma gondii E/SA immune response |
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