| 伊氏锥虫和媾疫锥虫蛋白组分的电泳分析 |
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| 引用本文： | 张浩吉,甄英凯,欧永春,王芳生,孙恩贵,刘俊华,张凤琴.伊氏锥虫和媾疫锥虫蛋白组分的电泳分析[J].中国兽医学报,1993(1). |
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| 作者姓名： | 张浩吉 甄英凯 欧永春 王芳生 孙恩贵 刘俊华 张凤琴 |
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| 作者单位： | 兽医大学军事兽医研究所
(张浩吉，甄英凯，欧永春，王芳生，孙恩贵，刘俊华)，兽医大学军事兽医研究所(张凤琴) |
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| 摘 要： | 应用SDS—PAGE和IEF电泳对伊氏锥虫和媾疫锥虫的蛋白组分进行了分析.在SDS—PAGE中,伊氏锥虫有21条带.媾疫锥虫有19条带,两种虫体的蛋白质区带在分子量40000～90000之间存在差异,尤其表现在表面糖蛋白上,伊氏锥虫为43000,媾疫锥虫为60000.在IEF电泳中,伊氏锥虫出现26条带,媾疫锥虫出现33条带,两种虫体的蛋白质区带在等电点4.8～5.6之间相同,而在5.6～7.0之间存在差异.本实验还对马媾疫锥虫的蛋白质进行了双相电泳分析,显示出86个多肽斑点.
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| 关 键 词： | 伊氏锥虫 媾疫锥虫 蛋白质 SDS—聚丙烯酰胺凝胶电泳 等电聚焦电泳 双相电泳 |
The Electrophoretic Analyses of the Protein Fractions of Trypanosoma evansi and T. equiperdum |
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| Zhang Haoji et al.The Electrophoretic Analyses of the Protein Fractions of Trypanosoma evansi and T. equiperdum[J].Chinese Journal of Veterinary Science,1993(1). |
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| Authors: | Zhang Haoji |
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| Institution: | Zhang Haoji et al. The Military Veterinary Institute,Veterinary College of PLA |
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| Abstract: | The protein fractions of Trypanosoma evansi and T. equiperdum were analysed bySDS-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). SDS-PAGE showed 21 and 19 bands in T. evansi and T. equiperdum, respectively. The difference be-tween the two species is present at variant surface glycoproteins (43 and 60 KD, respectively)and some other proteins with M. W. of 40-90 KD. IEF showed 26 protein bands in T. evansi and33 in T. equiperdum. The differences ranged from pH 5.6 to pH 7.0. In addition, two-dimen-sional electrophoresis was conducted and 86 discrete spots were observed in T. equiperdum. |
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| Keywords: | Trypanosoma evansi T equiperdum protein SDS-PAGE IEF two-dimensional electrophoresis |
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