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迟缓爱德华氏菌胞外产物的细胞毒性和动物致病性
引用本文:葛艳,陈怀青,陆承平.迟缓爱德华氏菌胞外产物的细胞毒性和动物致病性[J].中国兽医学报,2000,20(1):34-37.
作者姓名:葛艳  陈怀青  陆承平
作者单位:南京农业大学,动物医学院,江苏,南京,210095
基金项目:江苏省“九五”重点攻关项目!(BE964 2 7)
摘    要:分析了28株迟缓受德华氏菌(Edwardwiella tarda,Et)胞外产物(extracellular puodrcts,ECP)的致病性。将Et用覆有玻璃纸的TSA培养,18株(69.23%)的 ECP使HEp-2细胞产生病认,认圆,脱落。这种细胞毒作用能被ATCC15947株的ECP抗体作为探 针进行免疫转印分析,结果显示,不同来源的9株Et的ECP在 转印膜上出现3条共同蛋白条带,相对分子质量分别约14400、32、300、79、

关 键 词:迟缓爱德华氏菌  胞外产物  细胞毒性  致病性
修稿时间:1999-01-03

Cytotoxity and Pathogenicity in the Mice and Fish of Extracellular Products of Edwardsiella Tarda
GE Yan,CHEN Huai-qing,LU Cheng-ping.Cytotoxity and Pathogenicity in the Mice and Fish of Extracellular Products of Edwardsiella Tarda[J].Chinese Journal of Veterinary Science,2000,20(1):34-37.
Authors:GE Yan  CHEN Huai-qing  LU Cheng-ping
Abstract:The cytotoxity of extracellular products (ECP) of Edwardsiella tarda (Et) strains from different sources were investigated. E CP were prepared by a cellophane-overlaid agar method. The ECP of 18/26 Et stra ins had a cytotoxic effect on HEp-2 cells, which could be inhibitied by the ant iserum against ECP of ATCC15947. The ECP had no toxic effects to HEp-2 cells af ter being dealt with trypsin or heat. Western-blot of ECP probed with antisera against ECP of ATCC15947 strain showed that 9 virulent strains from different so urces had the same three antigenic proteins of 14 400, 32 300 and 79 400. The to xity of ECP of 5 selected Et strains was tested in the mice and fish (Xiphopho rus helleri) with the pathogenicity of cytotoxic ECP of 4 strains to mice and fish to be 75% and 67.5% respectively, while the ECP without cytotoxity had no v irulence to these animals.
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