| 猪肌生成抑制素（MSTN）cDNA的克隆 |
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| 引用本文： | 李慎涛,廖晓萍,等.猪肌生成抑制素（MSTN）cDNA的克隆[J].中国兽医学报,2001,21(1):31-34. |
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| 作者姓名： | 李慎涛 廖晓萍 |
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| 作者单位： | [1]解放军军需大学基础部，吉林长春130062 [2]华南农业大学，广东广州510642 |
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| 基金项目： | 国家自然科学基金资助项目! (30 0 70 5 6 3)，军队医药卫生青年基金项目! (98Q0 79) |
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| 摘 要： | 从猪的肌生成抑制素（MSTN）编码序列中设计引物，以军牧一号猪肌细胞总PNA为模板，利用RT－PCR和嵌套PCR技术，扩增出MSTN cDNA片段。该片段全长1277bp，包含猪MSTN基因的全部编码序列。将所得片段与pMD18-T载体连接，转化到JM109大肠杆菌中，成功地筛选到阳性克隆，其质粒测序结果与文献报道的一致。经EcoR Ⅰ和Pst Ⅰ酶解分析，cDNA片段与pMD18-T载本之间既有正向插入的克隆，也有反向插入的克隆，所得到的MSTN cDNA可用于原核和真核表达载体的构建。
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| 关 键 词： | 猪 肌生成抑制素 基因克隆 |
Cloning of Swine Myostatin Gene cDNA |
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| LI Shen tao ,SUN Bo xing ,OUYANG Hong sheng ,ZHANG Yu jing ,LIAO Xiao ping ,ZHANG Rui ,ZHANG Yong liang ,WU Wen jia.Cloning of Swine Myostatin Gene cDNA[J].Chinese Journal of Veterinary Science,2001,21(1):31-34. |
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| Authors: | LI Shen tao SUN Bo xing OUYANG Hong sheng ZHANG Yu jing LIAO Xiao ping ZHANG Rui ZHANG Yong liang WU Wen jia |
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| Institution: | LI Shen tao 1,SUN Bo xing 1,OUYANG Hong sheng 1,ZHANG Yu jing 1,LIAO Xiao ping 2,ZHANG Rui 1,ZHANG Yong liang 1,WU Wen jia 3 |
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| Abstract: | Total RNA of muscle cell was prepared from a new pig breed JunMu 1 and used in RT PCR to amplify myostatin(MSTN) gene cDNA.The resulted cDNA fragment with the expected size was cloned into vector pMD 18 T and subsequently subjected to restriction enzyme analysis and sequencing. The result showed that the cloned MSTN gene is 1 277 bp comprising the complete coding sequence and 99.5% homogenous to published data with replacement of 4 nucleotides causing only one amino acid changed.The expression of cloned MSTN gene in prokaryotic and eukaryotic system is under way. |
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| Keywords: | swine myostatin gene cloning |
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