猪肝棘球蚴超微结构和棘球蚴病病理形态学观察

对97例猪肝棘球蚴进行了系统的病理形态学观察,同时对其中3例作了扫描和透射电镜观察。病理学检查,均为单房型棘球蚴;采取囊液离心作头节检查,仅少部分有原头蚴。棘球蚴囊壁分为两层,内层(或胚层)较薄,伊红着染,外层为较厚的角质层,伊红淡染,呈无构造板层状结构。两层内无血管。PAS反应均为阳性。虫体周围常有变态反应性炎,形成寄生虫性肉穿肿,为本病的特征性病变。陈旧性肉芽肿中心无血管。肝组织是慢性间质性炎或肝硬化。透射电镜观察,胚层有很多生发层细胞呈指状突起的囊腔,其内有大量糖原颗粒和电子密度高的类核结构。胚层向角质层释放小颗粒样物质,角质层呈纤维网状。扫描电镜观察,胚层及内含物有生发囊、子囊和无机盐。角质层断面呈纤维网状,其外面有排列整齐的低柱状突起,形成有规则的皱褶,形如脑回状,表面密布较长的微绒毛。

AN OBSERVATION OF THE ULTRASTRUCTURE OF PORCINE HEPATIC ECHINOCOCCUS CYSTS AND PATHOMORPHOLOGICAL CHANGES OF THE DISEASE

Ninety-seven cases of porcine hepatic echinococciasis had beenstudied pathomorphologically and 3 of these examined hy scanning and trans-mitted electron-microscopy. The results obtained are as follows: All the Echino-coccus cysts observed are single capsule type. Only a few cases of cyst fluidscontain protoscolices after centrifugation. Its laminated wall is composed oftwo layers which are PAS positive, the endoderm is thinner and eosin staining,while the ectoderm is thicker, keratosic and amorphous and slightly stainedwith eosin. No blood vessels between two layers have been found. Aroundthe invading larvae allergic inflammation and parasitic granulomas usually ap-peared. These are characteristic lesions of the disease. The liver had chronicinterstitial hepatitis or hepatocirrhosis. There are no blood vessels within thecentres of old granulomas. By transmitted electronmicroscopy, the capsulesformed as finger-like projections from the germinal layer which lines thecyst are found. Within each of these capsules, numerous glycogen particlesand high electron-dense, nucleoid materials exist. Small particle-like materialsare eliminated from germinal layer to keratose layer. The keratose layer showsfibrous network appearance. By scanning electron-microscopy, daughter cysts,bloodcapsules and inotganic salts have been found. The transverse featureof keratose layer is fibrous network in appearance and the surface is covereddensely with longer microvilli. There are short cylindrical projections line uporderly on the surface, thus forming corrugations resembling the convolutionsof the brain.