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新城疫病毒强毒株的分离与鉴定
引用本文:范根成,朱万光,王永玲,王玉东,蒋贻海,张子春,刘相娥,吴延功,孙丰兰,杜元钊.新城疫病毒强毒株的分离与鉴定[J].中国兽医学报,1999,19(2):114-117.
作者姓名:范根成  朱万光  王永玲  王玉东  蒋贻海  张子春  刘相娥  吴延功  孙丰兰  杜元钊
作者单位:农业部动物检疫所,山东青岛,266032
摘    要:从发病率为100%、病死率为97%的鸡群中分离到1株病毒。该病毒凝集鸡红细胞的作用可被新城疫标准阳性血清所抑制,证明所分离的毒株为新城疫病毒。鸡胚半数致死量、最小致死量致死鸡胚的平均时间、1日龄雏鸡脑内致死指数、6周龄雏鸡静脉致死指数、血凝解脱及血凝素热稳定性等试验表明,分离毒为新城疫病毒强毒株,其毒力比标准强毒F48E8株还强。血凝抑制试验和中和试验表明,分离毒的血凝性与F48E8相同,但中和特性与F48E8有较大差异

关 键 词:新城疫病毒  强毒株  
修稿时间:1998-02-18

Isolation and Identification of High Virulent Newcastle Disease Virus
Fan Gencheng,Zhu Wanguang,Wang Yongling,Wang Yudong,Jiang Yihai,Zhang Zichun,Liu Xiange,Wu Yangong,Sun Fenglan,Du Yuanzhao.Isolation and Identification of High Virulent Newcastle Disease Virus[J].Chinese Journal of Veterinary Science,1999,19(2):114-117.
Authors:Fan Gencheng  Zhu Wanguang  Wang Yongling  Wang Yudong  Jiang Yihai  Zhang Zichun  Liu Xiange  Wu Yangong  Sun Fenglan  Du Yuanzhao
Abstract:One virus isolate was got from one chicken flock with the morbidity of 100% and mortality of 97%. The isolate revealed to be able to agglutinate chicken haemocytes with the agglutination reaction to be inhibited by Newcastle disease virus standard antiserum. The isolate was confirmed to be Newcastle disease virus based on 50% embryo lethal dose(ELD 50 ), mean death time (MDT) of chicken embryos, intracerebral pathogenicity index (ICPI) in 1 day old chicken, intravenous pathogenicity index (IVPI) in 6 week old chicken, dehaemagglutination and haemagglutinin heat stability. The isolate was proved more virulent than F 48 E 8 strain. The results of hemagglutination and neutralization tests indicated that the isolate was identical to in haemagglutination and quite different from the F 48 E 8 strain in neutralization property.
Keywords:Newcastle disease virus  high virulent strain  chicken  
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