猪细胞巨化病毒四川株MCP基因克隆及其编码蛋白的生物信息学分析

参照GenBank公布的仅有的日本株猪细胞巨化病毒较大衣壳蛋白（MCP）基因序列（登录号：AB051069）设计2对引物，采用分步克隆的方法，将PCMVMCP全序列克隆入pMD19-T载体进行测序，成功获得了pMD-MCP重组质粒，将所得序列录入到GenBank中（登录号：HQ025802）。测序结果表明，该MCP基因全长4017bp，共编码1338个氨基酸；与NCBI上公布的仅有的日本毒株MCP基因的核苷酸同源性为96．8％，氨基酸同源性为94．1％；进化分析显示：PCMVMCP基因与人疱疹病毒6型或7型的MCP基因亲缘关系较近；利用生物信息学软件对蛋白质结构特征进行分析，发现该蛋白含有59个潜在的磷酸化位点，潜在功能强大；亚细胞定位预测结果表明该蛋白主要存在于线粒体中和细胞质中并各占39．1％和26．1％，内质网占17．4％，高尔基体占8．7％，空泡和细胞核均占4．3％，表明PCMVMCP蛋白属于胞质蛋白，抗原区位集中于胞膜，有向胞质移动的趋势；另该成熟蛋白存在18个主要的抗原位点，将肽链经亲水性与抗原表位的共同分析，发现其肽链的c端极有可能分布有抗原决定簇。

Cloning MCP gene of porcine cytomegalovirus Sichuan strain and bioinformatics analysis of its coding protein

According to the only genomic sequence of PCMV MCP gene from Japan(GenBank accession number:AB051069),two pairs of specific primer were designed to amplify the fragment encoding PCMV MCP gene by PCR.These fragment was cloned into pMD19-T vector to sequence.The recombinant plasmid was designated as pMD-MCP successfully.The results indicated that length of PCR product of interest was 4 017 bp,encoding 1 338 amino acids in all.the alignment result showed that the homology of nucleotide was 96.8% and the homolo...