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| 猪瘟病毒重组E2蛋白PPA-ELISA抗体检测试剂盒的研制及应用 | |
| 引用本文： | 李娇,王金良,祖立闯,谢金文,沈志强.猪瘟病毒重组E2蛋白PPA-ELISA抗体检测试剂盒的研制及应用[J].中国兽医学报,2011,31(10). |
| 作者姓名： | 李娇 王金良 祖立闯 谢金文 沈志强 |
| 作者单位： | 1. 山东绿都生物科技有限公司,山东滨州,356600 2. 山尔省滨州畜牧兽医研究院,山东滨州,256600 3. 山东绿都生物科技有限公司,山东滨州356600／山尔省滨州畜牧兽医研究院,山东滨州256600 |
| 基金项目： | 山东省自主创新成果转化重大专项计划资助项目(2008ZHZX1A1103) |
| 摘 要： | 以纯化的猪瘟病毒（CSFV）重组E2蛋白为包被抗原,辣根过氧化物酶标记葡萄球菌A蛋白为二抗,组装了检测CSFV抗体的PPA-ELISA诊断试剂盒。该试剂盒与其他7种常见猪病毒（PRRSV、PPV、JEV、PCV-2、PEDV、PRV、TGEV）及牛病毒性腹泻病毒（BVDV）的阳性血清不发生交叉反应;批内重复性试验的变异系数小于5%,批间重复性试验的变异系数小于10%;与间接血凝试验相比较,符合率、敏感性和特异性分别为86.2%,85.4%,87.2%;与IDEXX ELISA试剂盒相比较,符合率、敏感性和特异性分别为91.3%,93.2%,88.7%。表明本试验研制的E2-PPA-ELISA抗体检测试剂盒具有良好的重复性、敏感性和特异性,将为CSFV的快速诊断、免疫猪群抗体水平监测和CSFV流行病学调查提供一种快速、简便的血清学诊断方法。 |
| 关 键 词： | 猪瘟病毒 重组E2蛋白 PPA-ELISA |
Development and application of PPA-ELISA for detecting antibodies to classical swine fever virus using recombinant E2 antigen |
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| LI Jiao,WANG Jin-liang,ZU Li-chuang,XIE Jin-wen,SHEN Zhi-qiang.Development and application of PPA-ELISA for detecting antibodies to classical swine fever virus using recombinant E2 antigen[J].Chinese Journal of Veterinary Science,2011,31(10). | |
| Authors: | LI Jiao WANG Jin-liang ZU Li-chuang XIE Jin-wen SHEN Zhi-qiang |
| Institution: | LI Jiao1,WANG Jin-liang2,ZU Li-chuang1,XIE Jin-wen2,SHEN Zhi-qiang1,2*(1.Shandong Lvdu Bio-Sciences & Technology Co.Ltd.,Binzhou,Shandong 256600,China,2.Shandong Binzhou Animal Science &Veterinary Medicine Academy,China) |
| Abstract: | To develop a method to detect the antibody of classical swine fever virus(CSFV),an indirect ELISA kit was developed using the purified recombinant E2 protein as coating antigen and HRP-SPA as the second antibody.The E2-PPA-ELISA kit showed no cross-reaction with the positive sera of other eight virus(PRRSV,PPV,JEV,PCV-2,PEDV,PRV,TGEV,BVDV).Coefficient of variability percent(C.V %) of intro-batch and inter-batch duplicative tests was less than 5% and 10%,respectively.Comparing with the indirect hemagglutinat... |
| Keywords: | classical swine fever virus recombinant E2 protein antigen PPA-ELISA |
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