| 抑制性削减技术研究CD3／CD28单抗共刺激人T淋巴细胞基因差异 |
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| 引用本文： | 李新平,陈曦,王丽娜,李创宏,张文龙,张淼涛.抑制性削减技术研究CD3／CD28单抗共刺激人T淋巴细胞基因差异[J].中国兽医学报,2011,31(11). |
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| 作者姓名： | 李新平 陈曦 王丽娜 李创宏 张文龙 张淼涛 |
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| 作者单位： | 西北农林科技大学动物医学院,陕西杨陵,712100 |
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| 基金项目： | 国家自然科学基金资助项目(30471250) |
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| 摘 要： | 为了研究参与人T细胞活化、增殖、信号转导的相关基因，以正常人初始T细胞作为对照样本，以抗人CD3抗体联合抗人CD28抗体激发的人T细胞作为试验样本，进行抑制性削减杂交。结果筛选到43个侯选克隆，对其单向测序后，经过与GenBank同源序列比较，证实了6个克隆参与了T细胞活化增殖和信号转导的下游通路，分别为CCND2、RhoA、IRF4、FKSG44、EBP、PRPPS2；13个克隆所可能编码的基因与T细胞活化、分裂增殖、分化有关，另外24个克隆在GenBank无法查到对应的同源基因，可能代表了新基因。对这些基因的进一步研究，有助于阐释T细胞活化、增殖的信号转导机制，为了解体内活化T细胞参与的生理和病理过程，具有一定的指导意义。
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| 关 键 词： | 抑制性削减杂 T细胞活化 信号转导 CD3抗体 CD28抗体 |
Study on differentially expressed genes in human T cells coactivated by anti-CD3 and anti-CD28 antibodies using SSH technique |
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| LI Xin-ping,CHEN Xi,WANG Li-na,LI Chuang-hong,ZHANG Wen-long,ZHANG Miao-tao.Study on differentially expressed genes in human T cells coactivated by anti-CD3 and anti-CD28 antibodies using SSH technique[J].Chinese Journal of Veterinary Science,2011,31(11). |
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| Authors: | LI Xin-ping CHEN Xi WANG Li-na LI Chuang-hong ZHANG Wen-long ZHANG Miao-tao |
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| Institution: | LI Xin-ping,CHEN Xi,WANG Li-na,LI Chuang-hong,ZHANG Wen-long,ZHANG Miao-tao(Animal Physiology Sector,Veterinary College,NorthWest A &F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | To study genes involved in human T cell proliferation,differentiation and signal transduction,a suppression subtractive hybridization(SSH) technique was developed to screen the specific(differentially expressed) transcrips of T cells coactivated with anti-CD3 and anti-CD28 antibodies serving as tester,and the normal T cells as driver in the experiments.This experiment revealed a total of 43 gene regulations.Only 6(13.9%) of these regulated genes had OMIM records annotated as T cell activation genes(CCND2,Rh... |
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| Keywords: | suppression subtractive hybridization activated T cell signal transduction anti-CD3 anti-CD28 |
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