| 牦牛胎盘滋养层细胞的分离培养 |
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| 引用本文： | 赵志文,樊江峰,刘犇,余四九.牦牛胎盘滋养层细胞的分离培养[J].中国兽医学报,2011,31(4). |
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| 作者姓名： | 赵志文 樊江峰 刘犇 余四九 |
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| 作者单位： | 甘肃农业大学动物医学院,甘肃兰州,730070 |
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| 基金项目： | 国家自然科学基金资助项目(30871903) |
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| 摘 要： | 采用联合消化液对妊娠8～10周龄的胎儿胎盘组织进行了消化,获得单细胞悬液,然后将滋养层细胞分离纯化,探索其培养的最适FBS浓度及最适pH,并观察其大体形态、胞核的特点、细胞角蛋白7(Cytokeratin,CK7)和波形蛋白(Vimentin,Vim)表达等。结果显示,牦牛滋养层细胞在pH6.8～7.0、20%FBS的DMEM/F12培养基条件下适宜传代培养;该细胞为上皮样细胞形态,呈片状铺展生长;细胞角蛋白染色阳性,波形蛋白染色阴性;阳性细胞率达90%,台盼蓝排斥试验显示活细胞率超过95%。
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| 关 键 词： | 牦牛 滋养层细胞 体外培养 |
Isolation and culture in vitro of yak placental trophoblastic cells |
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| ZHAO Zhi-wen,FAN Jiang-feng,LIU Ben,YU Si-jiu.Isolation and culture in vitro of yak placental trophoblastic cells[J].Chinese Journal of Veterinary Science,2011,31(4). |
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| Authors: | ZHAO Zhi-wen FAN Jiang-feng LIU Ben YU Si-jiu |
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| Institution: | ZHAO Zhi-wen1,FAN Jiang-feng,LIU Ben,YU Si-jiu*(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China) |
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| Abstract: | To estalish isolation and culture method of yak placental trophoblast cells,the present experiment was carried out by useing co-digestion of the fetal placental tissues of 8 to 10 week old-fetus,to obtain the single trophoblast cell suspension.The trophoblast cells was then isolated and purified.The optimal concentration of FBS and optimal pH were determined for culturing the trophoblast cells;meanwhile,the general morphology and nuclear characteristics of the trophoblast cells,and the expression of cytoker... |
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| Keywords: | yak trophoblastic cells culture in vitro |
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