| 免疫鸡群感染新城疫强毒后的排毒规律 |
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| 引用本文： | 文其乙,吴艳涛,刘秀梵,张如宽,焦新安,吴长新,彭大新,高崧.免疫鸡群感染新城疫强毒后的排毒规律[J].中国兽医学报,1999,19(1):15-17. |
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| 作者姓名： | 文其乙 吴艳涛 刘秀梵 张如宽 焦新安 吴长新 彭大新 高崧 |
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| 作者单位： | 扬州大学农学院动物医学系,江苏扬州,225009 |
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| 基金项目： | 国家“九五”攻关,江苏省“九五”攻关课题 |
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| 摘 要： | 将经过２次新城疫（ＮＤ）基础免疫的ＳＰＦ鸡，随机分成５组，每组８只，在６０日龄时分别用Ｆ４８Ｅ８、Ｔｅｘａｓ、Ｈｅｒｔ３３ＮＤ强毒和Ｌａｓｏｔａ弱毒经口腔接种０．２５ｍＬ／只，１２ｈ后采取泄殖腔棉拭，利用ＮＤ强毒快速诊断试剂盒进行排毒的动态监测，并在攻毒前期、中期、后期测定各试验组ＳＰＦ鸡的ＨＩ效价。试验第３天开始排毒，２０ｄ结束，其间在６～７ｄ和１１～１３ｄ出现２次排毒高峰。ＨＩ抗体在初期先是降低１个滴度，后期开始升高而且离散度不断增大。
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| 关 键 词： | 新城疫病毒 排毒规律 诊断试剂盒 ELISA |
| 修稿时间： | 1997-11-06 |
Dynamical Supervision on Shedding Virus in SPF Chicken Flocks Infected with Different Pathotypes of NDV |
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| Wen Qiyi,Wu Yantao,Liu Xiufan,Zhang Rukuan,Jiao Xinan,Wu Changxin,Peng Daxin,Gao Song.Dynamical Supervision on Shedding Virus in SPF Chicken Flocks Infected with Different Pathotypes of NDV[J].Chinese Journal of Veterinary Science,1999,19(1):15-17. |
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| Authors: | Wen Qiyi Wu Yantao Liu Xiufan Zhang Rukuan Jiao Xinan Wu Changxin Peng Daxin Gao Song |
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| Abstract: | A ELISA kit based on monoclonal antibodies was used to detect the dynamical change of shedding virus in SPF chicken flocks infected with different pathotypes of NDV (F48E8, Hert33, Texas, Lasota) and HI levels at early, middle and late stage post inoculation were detected by standard method. The results showed that only chicken flocks infected with virulent strains excreted viruses begining at three days of inoculation and ending on twentieth day. There were two peaks of shedding virus in chicken flocks infected with virulent strains of NDV. One peak appeared in 5 6 days of inoculation and another peak in 11 13 days of inoculation. While the chicken flocks infected with commonly mesogenin and lentogenic vaccine strains did not shed virus from cloacal. |
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| Keywords: | NDV shedding virus ELISA diagnostic kit |
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