| 牛病毒性腹泻-黏膜病病毒Changchun184株E2基因在卡介苗中的表达 |
| |
| 引用本文： | 杜锐,刁燕,韩俊友,张西臣,时坤.牛病毒性腹泻-黏膜病病毒Changchun184株E2基因在卡介苗中的表达[J].中国兽医学报,2009,29(7). |
| |
| 作者姓名： | 杜锐 刁燕 韩俊友 张西臣 时坤 |
| |
| 作者单位： | 1. 吉林农业大学,中药材学院,吉林,长春,130118
2. 吉林大学,畜牧兽医学院,吉林,长春,130062 |
| |
| 基金项目： | 国家自然科学基金，吉林省杰出青年科学基金，省部共建教育部重点实验室资助项目 |
| |
| 摘 要： | 在成功克隆牛病毒性腹泻一黏膜病痛毒Changchun184株E2基因的基础上,将E2基因与表达载体pMV261连接,构建了重组穿梭质粒pMV261-E2,后电转化到卡介苗中,获得了具有卡那霉素抗性的重组卡介苗,并对重组卡介苗进行菌落PCR鏊定.在45℃下热诱导E2基因在重组卡介苗中的表达,并对表达产物进行SDS-PAGE电泳和Wesern blotting 分析.结果证明,牛病毒性腹泻-黏膜病病毒Changchun184株E2基因在卡介苗中成功的表达.
|
| 关 键 词： | 牛病毒性腹泻-黏膜病 E2基因 重组卡介苗 |
Expressing of bovine viral diarrhea-mucosal disease virus Changchun184 strain of E2 gene in BCG |
| |
| DU Rui,DIAO Yan,HAN Jun-you,ZHANG Xi-chen,SHI Kun.Expressing of bovine viral diarrhea-mucosal disease virus Changchun184 strain of E2 gene in BCG[J].Chinese Journal of Veterinary Science,2009,29(7). |
| |
| Authors: | DU Rui DIAO Yan HAN Jun-you ZHANG Xi-chen SHI Kun |
| |
| Abstract: | E2 gene of BVDV Changchun 184 strain was cloned and inserted into the shuttle expression plasmid vector pMV261,the recombinant shuttle plasmid pMV261-E2 was constructed.Then pMV261-E2 was transformed into BCG successfully and obtained recombinant BCG which was resistive to kanamyein.The recombinant BCG were identifieated by PCR.E2 gene expression in recombinant BCG was induced in 45℃,then the SDS-PAGE and western blotting was used to analyze the expression product.The results indicated the BVDV E2 gene was expressd in BCG successfully. |
| |
| Keywords: | bovine viral diarrhea virus (BVDV) E2 gene recombinate BCG |
| 本文献已被 万方数据 等数据库收录！ |
|
