PCR方法检测奶牛粪便中鼠隐孢子虫

从含有鼠隐孢子虫卵囊的奶牛粪便中，直接提取 Ｄ Ｎ Ａ 用作 Ｐ Ｃ Ｒ 模板，用 １ 对人工合成的寡核苷酸作为 Ｐ Ｃ Ｒ 引物，扩增大小为 ５４０ ｂｐ 的特异片段。 Ｐ Ｃ Ｒ 产物经电泳鉴定，表明可从含隐孢子卵囊的奶牛粪便标本 Ｄ Ｎ Ａ 抽提物中扩增出目的片段，而其他几种寄生虫及阴性对照均不能扩增出特异片段。本方法的敏感性最低可检测到含卵囊 ４００ 个／ｍ Ｌ 的样本，具有敏感性高、特异性强的特点。

Detection of Cryptosporidium muris in Fecal Samples from Dairy Cattle by PCR

DNA extracted directly from fecal sample with Cryptosporidium muris oocysts in dairy cattle was used as the source of template for the PCR. A pair of oligonucleotide primers was synthesized and used to prime the amplification of a 540 bp target fragment were analyzed by electrophoresis in agarose gels stained with ethidium bromide. The results showed that the target fragment was present in DNA extracts of fecal samples from dairy cattle infected with C. muris, but not in DNA from any other parasites. The level of detection by this method was 400 oocysts/mL. This study showed that PCR was a highly sensitive and specific method for detction of C. murisand could serve as a valuable tool for clinical diagnosis and epidemilogical survey.