|
|||||
|
|
| 动物蓝舌病毒L2基因克隆及序列分析比较 | |
| 引用本文： | 张以芳,朱建波,刘建平,向文彬,信爱国,张念祖,殷震.动物蓝舌病毒L2基因克隆及序列分析比较[J].中国兽医学报,2002,22(6):536-538. |
| 作者姓名： | 张以芳 朱建波 刘建平 向文彬 信爱国 张念祖 殷震 |
| 作者单位： | 1. 云南农业大学,动物科技学院,云南,昆明,650201 2. 云南省热带亚热带动物病毒病重点实验室,云南,昆明,650201 3. 解放军军需大学军事兽医研究所,吉林,长春,130062 |
| 基金项目： | “九五”国家重点攻关项目 ( 96 0 5 0 2 0 10 3)，中国澳大利亚国际合作项目 ( ACIAR930 1)部分研究内容 |
| 摘 要： | 对 15株中国动物蓝舌病毒血清 1型 (BTV- 1)野毒株及 1株弱毒疫苗株 L 2基因进行了克隆和测序 ,并进行了系统进化分析。中国 BTV- 1型毒株分为 2个组 :分离年代最早的 Y86 3毒株 (1979年 )为 组 ;1996年以后分离的 14株毒株为 组。 2组之间的同源性是 91.6 %。 组又分为 4个谱系 ,疫苗株 Vac F45与原始株 V6 5 8分别属于 组的第 1和第 3谱系 ,核苷酸同源性 98.6 %。由此表明 ,中国 BTV- 1型毒株在自然进化及鸡胚传代驯化过程中 ,L2基因发生了遗传变异。 |
| 关 键 词： | 蓝舌病毒 L2基因 序列分析 |
| 文章编号： | 1005-4545(2002)06-0536-03 |
| 修稿时间： | 2001年9月27日 |
Cloning and Sequence Comparison of L2 Genes of Bluetongue Virus from China |
|
| ZHANG Yi-fang ,ZHU Jian-bo ,LIU Jian-ping ,XIANG Wen-bin ,XIN Ai-guo ,ZHANG Nian-zu ,YIN Zhen.Cloning and Sequence Comparison of L2 Genes of Bluetongue Virus from China[J].Chinese Journal of Veterinary Science,2002,22(6):536-538. | |
| Authors: | ZHANG Yi-fang ZHU Jian-bo LIU Jian-ping XIANG Wen-bin XIN Ai-guo ZHANG Nian-zu YIN Zhen |
| Institution: | ZHANG Yi-fang 1,ZHU Jian-bo 2,LIU Jian-ping 1,XIANG Wen-bin 2,XIN Ai-guo 2,ZHANG Nian-zu 2,YIN Zhen 3 |
| Abstract: | A portion of the 12 gene from 15 field strain isolates and 1 attenuated vaccine strain of Chinese BTV serotype 1,corresponding to nucleotide 854- 1 806 was amplified via RT-PCR,cloned and sequenced. Phylogenetic analysis was done on the amplified region of L2 gene of Chinese strains of BTV serotype 1.The L2 gene sequences segregated into two distinct monophyletic groups(A and B).Group A included Y863 strain isolated in 1979.Group B included 1 attenuated vaccine strain and 14 virus strains isolated after 1996.Strains of group B formed five lineage,vaccine strain and prototype strains of BTV were divided into different lineage,These results showed that L2 sequences of Chinese BTV strains of serotype 1 had genetic variation in procession of natural evolution and embryo culture. |
| Keywords: | bluetongue virus L2 gene sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |