番鸭细小病毒和鹅细小病毒生化及基因组特性的比较

番鸭细小病毒莆田株（MPV-P）和鹅细小病毒莆田株（GPV-P）分别感染的番鸭胚尿囊液经氯仿处理、PEG沉淀和Sepharose4B柱纯化。纯化样品在电镜下观察，均见到实心和空心2种病毒粒子。病毒粒子呈圆形，立体对称，无囊膜，直径为20-24nm。经SDS-PAGE分析，MPV和GPV均呈现3条结构蛋白带。MPV结构蛋白的相对分子质量约为VP1 89000、VP2 78000和VP3 61000，其中VP3为主要结构蛋白。GPV要相对分子质量与MPV有微小差别。MPV和GPV核酸均为单链DNA，长度约为51000bp。分别以MPV核酸和GPV核酸为模板，加到无引物的DNA聚合酶Ⅰ大片段合成体系中，合成产物经1%琼脂糖凝胶电泳，均可见长度约为5600bp的双链DNA带，证明MPV和GPV核酸的3'末端具有发夹结构。对这2种病毒核酸及经Klenow合成的双链核酸进行限制性内切酶分析，两者的酶切结果明显不同，表明MPV和GPV核酸在一级结构上存在明显差异。以上结果证明，MPV和GPV不但在致病性上有显著养异，而且在核酸结构上也有明显差异。

Comparison of Biochemical and Genomical Characteristics Between Muscovy Duck Parvovirus and Goose Parvovirus

The allantic fluids of duck embryo inoculated with muscovy duck parvovirus(putian strain) and goose parvovirus(putian stain) were extracted with chloroform,pricipitated with PEG and followed by sepharose 4B column chromatography.The purified MPV and GPV Contained full and empty non enveloped cosahedral viral particles with a diameter of 22 24 nm in size.SDS PAGE analysis revealed that MPV have 3 major structural polypeptides,they are VP 1 89 000 ,VP 2 78 000 and VP 3 61 000 ,and that of GPV are very little different from MPV.The nucleic acids of Both MPV and GPV are single stranded DNA with about 5 100 bp in size.The restriction endonuclease digestion showed the very diffenent pattern between MPV and GPV nucleic acids.The results indicated that significant differences exist between the genome primary structure of MPV and GPV.