| 上海地区出现猪繁殖与呼吸综合征和2型猪圆环病毒混合感染 |
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| 引用本文： | 李玉峰,王先炜,姜平.上海地区出现猪繁殖与呼吸综合征和2型猪圆环病毒混合感染[J].中国兽医学报,2003,23(5):442-444. |
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| 作者姓名： | 李玉峰 王先炜 姜平 |
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| 作者单位： | 南京农业大学,动物医学院,农业部动物疫病诊断和免疫重点实验室,江苏,南京,210095 |
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| 基金项目： | 中华农业科教基金资助项目 |
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| 摘 要： | 上海地区6个规模化猪场断奶后仔猪出现全身消耗性综合征，剖检的18头病仔猪都表现肺脏严重病变和淋巴结肿大出血。分别设计针对猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus，PRRSV)N蛋白和2型猪圆环病毒(porcine circovirus type2，PCV-2)部分基因的特异性PCR引物，通过RT—PCR和PCR技术从患病猪肺脏组织均扩增出PRRSV和PCV-2的特异基因片段。结合临床症状和流行病学调查，证实上海地区出现PRRSV和PCV-2的混合感染。
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| 关 键 词： | 上海地区 猪 繁殖呼吸综合征病毒 2型猪圆环病毒 混合感染 RT-PCR PCR 症状 流行病学 |
| 文章编号： | 1005-4545(2003)05-0442-02 |
| 修稿时间： | 2002年7月17日 |
Occurrence of Porcine Circovirus Type 2 and Porcine Reproductive and Respiratory Syndrome Virus in Shanghai Area |
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| LI Yu feng,WANG Xian wei,JIANG Ping.Occurrence of Porcine Circovirus Type 2 and Porcine Reproductive and Respiratory Syndrome Virus in Shanghai Area[J].Chinese Journal of Veterinary Science,2003,23(5):442-444. |
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| Authors: | LI Yu feng WANG Xian wei JIANG Ping |
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| Institution: | LI Yu feng,WANG Xian wei,JIANG Ping * |
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| Abstract: | Eighteen piglets from 6 farms during outbreaks of postweaning multisystemic wasting syndrome(PMWS) in Shanghai,were investigated.The clinical ill piglets had severed lesions in lung and lymphoid tissue.Both PCR and RT PCR were used to detect porcine circovirus type 2(PCV 2) and porcine reproductive and respiratory syndrome virus(PRRSV),respectively.The results showed that PCV 2 and PRRSV infection were detected in all the ill piglets.PCV 2 and PRRSV could be considered as the possible agent involved in the pathogenesis of the syndrome. |
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| Keywords: | PRRSV PCV 2 PCR |
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